Human Oct-4A APC-conjugated Antibody

Catalog #: IC6344A Datasheet / COA / SDS

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IC6344A

Detection of Oct‑4A in BG01V Human Cells by Flow Cytometry.

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Human Oct-4A APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Oct-4A in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 653108

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Oct-4A
Met1-Glu135
Accession # NP_002692

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

BG01V human embryonic stem cells or iBJ6 iPSC cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Oct‑4A in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Oct-4A APC-conjugated Monoclonal Antibody (Catalog # IC6344A, filled histogram) or isotype control antibody (IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Flow Cytometry

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Detection of Oct‑4A in iPSCs by Flow Cytometry. iPSCs were stained with Mouse Anti-Human Oct‑4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-4A

Oct-3/4, alternately Oct-3 or Oct-4, is POU5F1 (POU domain containing, class 5, transcription factor 1), a 360 amino acid (aa) transcription factor that is expressed in totipotent embryonic stem and germ cells. The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct-4B, and Oct-4B1) and generates four protein isoforms by alternative splicing and alternative translation initiation. Oct-4A expression is restricted to embryonic stem (ES) and embryonic carcinoma (EC) cells and is believed to be the transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. In contrast, Oct-4B/4B1 can be detected in various nonpluripotent cell types and cannot sustain ES cell pluripotency and self-renewal.

Long Name

Octamer-binding Transcription Factor 4A

Entrez Gene IDs

5460 (Human); 18999 (Mouse)

Alternate Names

OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4A; Oct-4A