Human OLT-2/TARM1 Antibody

Catalog #: MAB10059 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB10059-100
MAB10059-SP

Detection of OLT-2/TARM1 in Peripheral Blood Granulocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human OLT-2/TARM1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human OLT-2/TARM-1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2564C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human OLT-2/TARM-1
Met1-Val235
Accession # B6A8C7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Peripheral blood granulocytes or U937 human histiocytic lymphoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of OLT-2/TARM1 in Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rabbit anti-Human OLT-2/TARM1 Monoclonal Antibody (Catalog # MAB10059, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Phycoerythrin-conjugated Secondary Antibody (F0110). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Flow Cytometry

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Detection of OLT-2/TARM1 on U937 Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Rabbit anti-Human OLT-2/TARM1 Monoclonal Antibody (MAB10059, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Phycoerythrin-conjugated Secondary Antibody (F0110). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OLT-2/TARM1

T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4⁺ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

References

Radjabova, V., et al. (2015) J Immunol. 195:3149.

Long Name

T-cell-interacting Activating Receptor on Myeloid Cells 1

Entrez Gene IDs

441864 (Human); 245126 (Mouse); 499065 (Rat)

Alternate Names

OLT2; OLT-2/TARM1; OSCAR-Like Transcript-2 Protein; T Cell-Interacting, Activating Receptor On Myeloid Cells 1; T Cell-Interacting, Activating Receptor On Myeloid Cells; TARM1; T-Cell-Interacting, Activating Receptor On Myeloid Cells Protein 1