Human Ornithine Carbamoyltransferase/OTC Antibody

Catalog #: MAB9918 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9918-100
MAB9918-SP

Ornithine Carbamoyltransferase/OTC in Human Liver.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ornithine Carbamoyltransferase/OTC Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ornithine Carbamoyltransferase/OTC in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 990421

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing human Ornithine Carbamoyltransferase/OTC
Accession # P00480

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Ornithine Carbamoyltransferase/OTC in Human Liver. Ornithine Carbamoyltransferase/OTC was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Ornithine Carbamoyltransferase/OTC Monoclonal Antibody (Catalog # MAB9918) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ornithine Carbamoyltransferase/OTC

Human Ornithine transcarbamylase (OTC) is a 354 amino acids, 40 KDa enzyme that catalyzes the reaction between carbamoyl phosphate (CP) and ornithine (Orn) to form citrulline (Cit) and phosphate (Pi). OTC is a trimer. The monomer unit has a CP-binding domain and an amino acid-binding domain. The active sites are located at the interface between the protein monomers.

Entrez Gene IDs

5009 (Human); 18416 (Mouse); 25611 (Rat)

Alternate Names

EC 2.1.3; EC 2.1.3.3; MGC129967; MGC129968; MGC138856; OCTD; Ornithine Carbamoyltransferase; ornithine carbamoyltransferase, mitochondrial; Ornithine Transcarbamylase; OTC; OTCase