Human Osteocalcin Antibody

Catalog #: MAB113272 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB113272-100
MAB113272-SP

Detection of Osteocalcin in SAOS-2 cells by Flow Cytometry.

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All Osteocalcin Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Osteocalcin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Osteocalcin in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 582014

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Osteocalcin/KLH containing peptide
Accession # P02818

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

SAOS-2 cells fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

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Detection of Osteocalcin in SAOS-2 cells by Flow Cytometry. SAOS-2 cells were stained with Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB113272, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteocalcin

Osteocalcin, also known as Bone gamma -Carboxyglutamic Acid Protein, is a secreted protein whose expression is restricted to cells of the osteoblast lineage (1). It has been frequently used as a marker for osteoblast lineage cells.

References

Lian, J.B. et al. (1999) Vitamin. Horm. 55:443.

Long Name

Bone gamma-Carboxyglutamate [gla] Protein

Entrez Gene IDs

632 (Human); 12096 (Mouse); 25295 (Rat)

Alternate Names

BGLAP; BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein; OC; OCN; Osteocalcin