Human Osterix/Sp7 MAb (Clone 764746)

Catalog #: MAB75471 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB75471-100
MAB75471-SP

Detection of Human Osterix/Sp7 by Western Blot.

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All Osterix/Sp7 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Osterix/Sp7 MAb (Clone 764746) Summary

Species Reactivity

Human

Specificity

Detects human Osterix/Sp7 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 764746

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Osterix/Sp7
Met19-Leu288
Accession # Q8TDD2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Osterix/Sp7 by Western Blot. Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB75471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Osterix/Sp7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osterix/Sp7

Osterix, also known as Sp7, is an approximately 45 kDa transcription factor that is required for osteogenesis and bone homeostasis. Osterix cooperates with BMP-6 in the differentiation of osteoblasts from mesenchymal stem cells by regulating the transcription of several genes involved in osteoblast differentiation and function (i.e. SATB2, Collagen V, and SOST). The transcription of Osterix is induced by BMP-2, IGF-I, and parathyroid hormone, while its activity is regulated by Akt and p38 MAPK-mediated phoshorylation. Osterix contains a transactivation domain (aa 141-210) and three zinc finger domains (aa 294-318, aa 324-348, and aa 354-376). Alternative splicing generates a short isoform that lacks the N-terminal 18 amino acids. Within aa 19-288, human Osterix shares 94% aa sequence identity with mouse and rat Osterix.

Long Name

Sp7 Transcription Factor

Entrez Gene IDs

121340 (Human); 170574 (Mouse); 300260 (Rat)

Alternate Names

MGC126598; Osterix; OSX; OSXosterix; Sp7 transcription factor; Sp7; transcription factor Sp7; Zinc finger protein osterix