Human OX40/TNFRSF4 Antibody

Catalog #: MAB10542 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10542-100
MAB10542-SP

Detection of OX40/TNFRSF4 in HEK293 Human Cell Line Transfected with Human OX40/TNFRSF4 and eGFP by Flow Cytometry.

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Human OX40/TNFRSF4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human OX40/TNFRSF4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 977960

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human OX40/TNFRSF4
Leu29-Ala216
Accession # P43489

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of OX40/TNFRSF4 in HEK293 Human Cell Line Transfected with Human OX40/TNFRSF4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human OX40/TNFRSF4 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10542) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OX40

OX40 (CD134; TNFRSF4) is a T cell co‑stimulatory molecule of the TNF receptor superfamily that coordinates with other membrane-bound co‑stimulators such as CD28, CD40, CD30, CD27 and 4-1BB (1‑3). OX40 is expressed on naïve CD4⁺ T cells only after engagement of the TCR by antigen presenting cells (APC; dendritic and B cells), and co‑stimulation by CD40/CD40 ligand and CD28/B7. It is maximal at 2‑5 days post activation, or 4 hours post reactivation of memory T cells (3‑6). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that has four TNFR-Cys repeats and an O-glycosylated hinge region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (3). The ECD of human OX40 shows 71%, 68%, 67%, 64% and 64% aa identity with feline, canine, rabbit, mouse and rat OX40 ECD, respectively. Engagement of OX40 on activated CD4⁺ T cells by OX40 ligand on activated dendritic cells promotes T cell survival and proliferation, prolongs the immune response, and enhances the number of cells making the transition from effector to memory T cells (1‑6). OX40 signal transduction includes binding TNF receptor-associated factors (TRAFs), and activating NF kappa B and PI3 kinase to enhance expression of cytokines, antiapoptotic Bcl-2 family members, survivin and the chemokine receptor CXCR5 (5‑8). CXCR5 promotes T cell migration to germinal centers to deliver B cell help (5). Studies using knockout or transgenic mice, and agonistic or blocking antibodies, show that OX40/OX40L interaction is critical for establishing or reactivating memory T cells and breaking immune tolerance (9). Blockade of OX40 engagement is efficacious in animal models of allergic airway inflammation, graft-versus-host disease and autoimmune disease (10‑14).

References

Salek-Ardakani, S. and M. Croft (2006) Vaccine 24:872.
Hori, T. (2006) Int. J. Hematol. 83:17.
Latza, U. et al. (1994) Eur. J. Immunol. 24:677.
Murata, K. et al. (2000) J. Exp. Med. 191:365.
Fillatreau, S and D. Gray (2003) J. Exp. Med. 197:195.
Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
Rogers, P.R. et al. (2001) Immunity 15:445.
Song, J. et al. (2005) Immunity 22:621.
Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
Jember, A. G. et al. (2001) J. Exp. Med. 193:387.
Demirci, G. et al. (2004) J. Immunol. 172:1691.
Blazar, B.R. et al. (2003) Blood 101:3741.
Higgins, L.M. et al. (1999) J. Immunol. 162:486.

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Alternate Names

ACT-135; ACT35 antigen; ACT35ATC35 antigen; CD134 antigen; CD134; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40; OX40L receptor; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; TNFRSF4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen