Human P-Cadherin PerCP-conjugated Antibody

Name: Human P-Cadherin PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB861C
Price: 365 USD

Catalog #: FAB861C Datasheet / COA / SDS

Newer Version Available: FAB861G

NEW

Discontinued Product

FAB861C has been discontinued and is replaced by FAB861G.

Detection of P‑Cadherin in A431 Human Cell Line by Flow Cytometry.

2 Images

All P-Cadherin Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human P-Cadherin PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human P-Cadherin in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) Cadherin‑8, recombinant mouse P-Cadherin, or rhVE-Cadherin.

Source

Monoclonal Mouse IgG₁ Clone # 104805

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human P-Cadherin
Asp108-Gly654
Accession # CAA45177

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Knockout Validated

P-Cadherin is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in P-Cadherin knockout A431 cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of P‑Cadherin in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human P-Cadherin PerCP-conjugated Monoclonal Antibody (Catalog # FAB861C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated

P-Cadherin Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

View Larger

P-Cadherin Specificity is Shown by Flow Cytometry in Knockout Cell Line. P-Cadherin knockout A431 human epithelial carcinoma cell line was stained with PerCP-conjugated Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # FAB861C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). No staining in the P-Cadherin knockout A431 cell line was observed. Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: P-Cadherin

Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.

References

Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
Overduin, M. et al. (1995) Science 267:386.
Takeichi, M. (1991) Science 251:1451.
Nose, A. et al. (1987) EMBO J. 6:3655.

Long Name

Placental Cadherin

Entrez Gene IDs

1001 (Human); 12560 (Mouse); 116777 (Rat)

Alternate Names

CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin