Human P2X7/P2RX7 Antibody

Catalog #: MAB97903 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB97903-100
MAB97903-SP

Detection of P2X7/P2RX7 in Hek 293 cells transfected with Human P2RX7 peptide and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human P2X7/P2RX7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human P2X7/P2RX7 in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1058613

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human P2X7/P2RX7 synthetic peptide
Accession # Q99572

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Hek 293 cells transfected with Human P2RX7 peptide and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of P2X7/P2RX7 in Hek 293 cells transfected with Human P2RX7 peptide and eGFP by Flow Cytometry. Hek293 cells transfected with Human P2RX7 peptide and eGFP were stained with either (A) Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB97903) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2X7/P2RX7

Human P2X purinoceptor 7 (aka P2X7) is a 595 aminoacids (aa) protein encoded by the P2RX7 gene. P2X7 receptors belong to the family of ATP-gated ion channels, and their activity can be found in cells of hemopoietic lineage including macrophages, microglia, and certain lymphocytes. They mediate the influx of Ca2+ and Na+ and the release of pro-inflammatory cytokines. At least 8 different human P2X7 isoforms formed by alternative splicing have been reported, ranging from 128 aa to 595 aa. The P2X7 subunits can form homomeric receptors only with a typical P2X receptor structure.

Long Name

P2X Purinergic Receptor 7

Entrez Gene IDs

5027 (Human); 18439 (Mouse); 29665 (Rat)

Alternate Names

ATP receptor; MGC20089; P2RX7; P2X purinoceptor 7; P2X7; P2X7P2X7 receptor; P2X7R; P2Z receptor; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7 variant A; Purinergic receptor