Human P2Y11/P2RY11 Antibody

Catalog #: MAB9305 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9305-100
MAB9305-SP

Detection of P2Y11/P2RY11 in HEK293 Human Cell Line Transfected with Human P2Y11/P2RY11 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human P2Y11/P2RY11 Antibody Summary

Species Reactivity

Human

Specificity

Stains human P2Y11/P2RY11 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 505214

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human P2Y11/P2RY11
Accession # Q96G91

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of P2Y11/P2RY11 in HEK293 Human Cell Line Transfected with Human P2Y11/P2RY11 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human P2Y11/P2RY11 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human P2Y11/P2RY11 Monoclonal Antibody (Catalog # MAB9305) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2Y11/P2RY11

P2RY11 belongs to the family of G protein-coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. P2RY11 is coupled to the stimulation of thephosphoinositide and adenylyl cyclase pathways and behaves as a selective purinoceptor. Naturally occuring read-through transcripts,resulting from intergenic splicing between P2RY11 and an immediately upstream gene (PPAN, encoding peter pan homolog), have been found. Variants in the P2RY11 gene have been associated with narcolepsy.

Long Name

P2Y Purinergic Receptor 11

Entrez Gene IDs

5032 (Human); 235036 (Mouse); 298699 (Rat)

Alternate Names

P2RY11; P2Y11; P2Y11P2Y purinoceptor 11; purinergic receptor P2Y, G-protein coupled, 11; purinergic receptor P2Y11