Human p38 beta /MAPK11 Antibody

Catalog #: MAB3274 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3274
MAB3274-SP

p38 beta /MAPK11 in Jurkat Human Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human p38 beta /MAPK11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human p38 beta /MAPK11 in direct ELISAs. No cross-reactivity with recombinant human p38 alpha, gamma, or delta is observed.

Source

Monoclonal Mouse IgG_2B Clone # 280071

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human p38 beta /MAPK11 synthetic peptide
EPPKPPGSLEIEQ
Accession # NP_002742

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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p38 beta /MAPK11 in Jurkat Human Cell Line. p38 beta /MAPK11 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human p38 beta /MAPK11 Monoclonal Antibody (Catalog # MAB3274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 beta/MAPK11

The p38 mitogen-activated protein kinases (p38 MAP kinases) are a family of four related Ser/Thr kinases responsive to pro-inflammatory cytokines and environmental stresses, including ionizing radiation, oxidative stress, and osmotic shock. Each family member, p38 alpha, p38 beta, p38δ, and p38 gamma, is activated by dual Thr and Tyr phosphorylation within a Thr-Gly-Tyr motif residing in the kinase activation loop. For p38 beta, also known as p38-2, stress-activated protein kinase 2B (SAPK2B), and MAPK11, this dual phosphorylation occurs at Thr180/Tyr182.

Long Name

Mitogen-Activated Protein Kinase 11

Entrez Gene IDs

5600 (Human); 19094 (Mouse); 689314 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.24; MAP kinase 11; MAP kinase p38 beta; MAPK 11; MAPK11; mitogen-activated protein kinase 11; Mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; MPK11; p38 beta; p38b; p38beta; P38BETA2; PRKM11; SAPK2B; SAPK2p38-2P38B; Stress-activated protein kinase 2; stress-activated protein kinase-2; stress-activated protein kinase-2b