Human p70 S6 Kinase beta Antibody

Catalog #: AF2987 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2987
AF2987-SP

Detection of Human p70 S6 Kinase beta by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human p70 S6 Kinase beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human p70 S6 Kinase beta in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human p70 S6 Kinase is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human p70 S6 Kinase beta
Ala2-Ser410
Accession # Q9UBS0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human p70 S6 Kinase beta by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p70 S6 Kinase beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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p70 S6 Kinase beta in MCF‑7 Human Cell Line. p70 S6 Kinase beta was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p70 S6 Kinase beta

P70S6K beta (p70 ribosomal S6 kinase beta-2; also RPS6KB2, S6K beta, S6K beta 2, S6K2 and STK14B) is a monomeric, 54 kDa member of the AGC family of Ser/Thr kinases. It is widely expressed, and found predominately in the nucleus. Although it was initially reported to phosphorylate the ribosomal S6 protein, p70S6K beta /S6K beta 2 is now known to also phosphorylate RafB (cell survival), hnRNP (cell proliferation), p300/CBP (transcription) and CDC42 (cytoskeleton stability). Human p70S6K beta /S6K beta 2 is 482 amino acids in length. It contains a protein kinase domain (aa 67-328), a C-terminal regulatory region (aa 329-399) that contains an NLS (aa 384-389), and a Pro-rich segment (aa 411-482) that likely interacts with SH3 domain-containing proteins. Phosphorylation/dephosphorylation events and sumoylation regulate p70S6K beta activity. In addition to the 54 kDa form, there is a 56-60 kDa isoform that possesses a 13 aa addition at the N-terminus, plus an isoform that shows a deletion of aa 267-270. Over aa 1-410, human p70S6K beta 2 shares 95% and 75% aa identity with mouse p70S6K beta 2and human p70S6K beta 1, respectively.

Entrez Gene IDs

6199 (Human); 58988 (Mouse); 361696 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.1; KLS; p70 ribosomal S6 kinase beta; p70 S6 Kinase beta; p70 S6KB; p70 S6K-beta; p70(S6K)-beta; p70-beta; P70-beta-1; P70-beta-2; p70-S6K 2; P70S6K2; p70S6Kb; ribosomal protein S6 kinase beta-2; ribosomal protein S6 kinase, 70kD, polypeptide 2,70 kDa ribosomal protein S6 kinase 2; ribosomal protein S6 kinase, 70kDa, polypeptide 2; RPS6KB2; S6K2; S6K-beta; S6K-beta2; S6K-beta-2; serine/threonine-protein kinase 14 beta; Serine/threonine-protein kinase 14B; SRK; STK14B; STK14BS6 kinase-related kinase