Human Pancreatic Polypeptide/PP Antibody

Catalog #: MAB6297 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6297
MAB6297-SP

Detection of Human Pancreatic Polypeptide by Western Blot.

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Human Pancreatic Polypeptide/PP Antibody Summary

Species Reactivity

Human

Specificity

Detects Pancreatic Polypeptide/PP in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 548301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Pancreatic Polypeptide/PP
Ala30-Leu95
Accession # P01298

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Pancreatic Polypeptide by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human Pancreatic Polypeptide Monoclonal Antibody (Catalog # MAB6297) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pancreatic Polypeptide at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pancreatic Polypeptide/PP

Pancreatic Polypeptide (PP) is an 11 kDa (calculated), unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence. PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake. The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.

Entrez Gene IDs

5539 (Human)

Alternate Names

INN; Obinepitide; pancreatic polypeptide (ppy); pancreatic polypeptide PNPPP; pancreatic polypeptide Y; Pancreatic Polypeptide; pancreatic prohormone; PNP; PP; PPY