Human Pancreatic Polypeptide/PP Antibody

Catalog #: AF6297
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6297
AF6297-SP

Pancreatic Polypeptide/PP in Human Pancreas.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pancreatic Polypeptide/PP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pancreatic Polypeptide/PP in direct ELISAs. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse PPY is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Pancreatic Polypeptide/PP
Ala30-Leu95
Accession # P01298

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Pancreatic Polypeptide/PP in Human Pancreas. Pancreatic Polypeptide/PP was detected in immersion fixed paraffin-embedded sections of human pancreas using Human Pancreatic Polypeptide/PP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6297) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pancreatic Polypeptide/PP

Pancreatic polypeptide (PP) is an ~4 kDa, unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence. PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake. The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.

Entrez Gene IDs

5539 (Human)

Alternate Names

INN; Obinepitide; pancreatic polypeptide (ppy); pancreatic polypeptide PNPPP; pancreatic polypeptide Y; Pancreatic Polypeptide; pancreatic prohormone; PNP; PP; PPY

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Pancreatic Polypeptide/PP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1