Human Pax5/BSAP Antibody

Catalog #: AF3487 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3487
AF3487-SP

Detection of Human Pax5/BSAP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pax5/BSAP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pax5/BSAP in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Pax2, rhPax3, and rhPax4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Pax5/BSAP
Thr141-His391
Accession # Q02548

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

Immersion fixed human peripheral blood mononuclear cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Pax5/BSAP by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Pax5/BSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3487) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pax5/BSAP at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Pax5/BSAP in Human Tonsil. Pax5/BSAP was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human Pax5/BSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3487) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax5/BSAP

Pax5, also known as BSAP (B-cell-specific transcription factor) is a 42 kDa protein belonging to the paired box transcription factor family. It is a developmental regulator that is important for B-cell lineage commitment and development. Human Pax5 is a 391 amino acid (aa) protein containing the paired DNA-binding domain at the N‑terminal region. Several alternatively spliced isoforms with altered C‑terminal regions and possessing different transactivation properties have been described. All isoforms share the N‑terminal 235 aa with full-length Pax5. Human Pax5 shares 99%, 97% and 94% aa sequence identity with mouse, bovine and canine Pax5, respectively.

Long Name

Paired Box Gene 5/B-cell Specific Activator Protein

Entrez Gene IDs

5079 (Human); 18507 (Mouse)

Alternate Names

B cell specific activator protein; B-cell-specific transcription factor; BSAP; BSAPB-cell lineage specific activator; paired box 5; paired box gene 5 (B-cell lineage specific activator protein); paired box gene 5 (B-cell lineage specific activator); paired box homeotic gene 5; paired box protein Pax-5; Pax5; transcription factor PAX 5