Human PD-ECGF/Thymidine Phosphorylase Antibody

Catalog #: MAB1646 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1646
MAB1646-SP

All PD-ECGF/Thymidine Phosphorylase Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PD-ECGF/Thymidine Phosphorylase Antibody Summary

Species Reactivity

Human

Specificity

Detects human PD-ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 27917

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PD-ECGF/Thymidine Phosphorylase
Ala11-Gln482
Accession # P19971

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human PD-ECGF/Thymidine Phosphorylase (Catalog # 229-PE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-ECGF/Thymidine Phosphorylase

PD-ECGF, also known as Thymidine Phosphorylase and Gliostatin, is produced by placenta, platelets, liver, lung, spleen, lymph nodes, and peripheral lymphocytes. It is overexpressed by many tumors in response to chemical or physical stress. PD-ECGF promotes endothelial cell proliferation and chemotaxis and promotes angiogenesis. PD-ECGF is a key enzyme in the pyrimidine nucleoside salvage pathway. It also metabolizes and inactivates chemotherapeutic drugs such as 5‑fluorouracil and its derivatives. The human PD‑ECGF cDNA encodes a 482 amino acid (aa) polypeptide with a 10 aa propeptide. The protein is localized mostly within the producer cells. N-terminal truncation, resulting in proteins lacking 10 aa and 6 aa, has been observed in PD-ECGF purified from platelets and placenta, respectively. In solution, PD-ECGF exists as a non-disulfide linked homodimer.

References

Haraguchi, M. et al. (1994) Nature 368:198.
Toi, M. et al. (2005) Lancet Oncol. 6:158.
Akiyama, S. et al. (2004) Canc. Sci. 95:851.

Long Name

Platelet-derived Endothelial Cell Growth Factor

Entrez Gene IDs

1890 (Human); 72962 (Mouse)

Alternate Names

dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP