Human PDZD11 Antibody

Catalog #: AF5977 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5977
AF5977-SP

Detection of Human PDZD11 by Western Blot.

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All PDZD11 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDZD11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDZD11 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human PDZD2 and recombinant mouse PDZD2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PDZD11
Met1-His140
Accession # Q5EBL8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PDZD11 by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PDZD11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5977) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDZD11 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDZD11

PDZD11 (PDZ domain-containing protein 11; also AIPP1a and PISP) is a 17 kDa cytosolic protein that bears resemblance to members of the MALS/VELIS family of proteins. It is ubiquitously expressed, and appears to target calcium and copper ATPases to basolateral cell membranes. Human PDZD11 is 140 amino acids (aa) in length. It contains but one PDZ domain (aa 47-129) that apparently interacts with the C-terminus of partner proteins. One alternative start site exists that is 32 aa upstream of the standard site. This generates the AIPP1b isoform. Full-length human PDZD11 shares 97% aa identity with mouse PDZD11.

Long Name

PDZ Domain Containing 11

Entrez Gene IDs

51248 (Human); 72621 (Mouse); 302422 (Rat)

Alternate Names

AIPP1; ATPase-interacting PDZ protein; PDZ domain containing 11; PDZ domain-containing protein 11; PDZD11; PDZK11; PISP