Human Peptide YY Antibody

Catalog #: MAB94563 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB94563-100
MAB94563-SP

Detection of Human Peptide YY by Simple WesternTM.

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All Peptide YY Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Peptide YY Antibody Summary

Species Reactivity

Human

Specificity

Detects Peptide YY in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2581A

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Peptide YY synthetic peptide
Accession # P10082

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Simple Western

20 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western

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Detection of Human Peptide YY by Simple Western^TM. Simple Western lane view shows lysates of human colon tissue and human small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Peptide YY at approximately 3 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94563). This experiment was conducted under reducing conditions and using the 2-40. kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peptide YY

Peptide YY (PYY), a secretory peptide, is expressed by L cells (enteroendocrine cells) in the small and large intestine, Islet of Langerhans cells, testis, and in some central and peripheral neurons. Because PYY is released from L cells after feeding, it is thought to produce a satiety signal. While the regulation of appetite by PYY and GLP-1 have been studied extensively, recent evidence supports a role for PYY in Islet of Langerhans beta cell survival. PYY secretion is also modulated by gut microbiota, and levels of colonic PYY have reduced in rats with DSS-induced colitis, and in humans with inflammatory bowel disease (IBD), while circulating PYY increased in IBD.

Entrez Gene IDs

5697 (Human); 217212 (Mouse); 287730 (E. faecalis)

Alternate Names

Peptide tyrosine tyrosine; Peptide YY; Peptide-YY; PYY; PYY1; PYY-I