Human Perforin Antibody

Catalog #: MAB11298 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11298-100
MAB11298-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Perforin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Perforin in direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2771C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human Perforin
Pro22-Trp555
Accession # P14222

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Perforin in Human Tonsil. Perforin was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Rabbit Anti-Human Perforin Monoclonal Antibody (Catalog # MAB11298) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Perforin

Perforin is a pore forming, cytolytic protein that is a component of cytolytic T-cell and NK cell granules. Perforin activates clathrin- and dynamin-dependent endocytosis, which removes perforin and granzymes from the plasm membrane to early endosomes, preserving outer membrane integrity. Perforin facilitates granzyme uptake and avoids the proinflammatory necrotic death of a membrane-damaged cell. Defects in the Perforin gene results in Familial hemophagocytic lymphohistiocytosis (FHL). Perforin-based effector systems are involved not only in the lysis of abnormal cells but also in the down-regulation of cellular immune activation.

Long Name

Perforin 1 (Pore Forming Protein)

Entrez Gene IDs

5551 (Human); 18646 (Mouse); 50669 (Rat)

Alternate Names

Cytolysin; FLH2; HPLH2; HPLH2lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; P1PFN1; perforin 1 (pore forming protein); Perforin; perforin-1; PFP; PFPcytolysin; PRF1