Human Phospho-CrkL (Y207) Antibody

Catalog #: MAB6910 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6910
MAB6910-SP

Detection of Human Phospho-CrkL (Y207) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-CrkL (Y207) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Phospho-CrkL (Y207) in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 668915

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human CrkL Y207 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-CrkL (Y207) by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line untreated (-) or treated (+) with 300 U/mL CIP for 60 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CrkL (Y207) Monoclonal Antibody (Catalog # MAB6910) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-CrkL (Y207) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CrkL

CrkL is a widely-expressed 39 kDa adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and the immune response. Furthermore, this adaptor protein plays an essential role in Chronic Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein

Entrez Gene IDs

1399 (Human); 12929 (Mouse); 287942 (Rat)

Alternate Names

CrkL; crk-like protein; pCRKL; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian)-like