Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

Catalog #: MAB1825
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1825
MAB1825-SP

Detection of Human and Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot.

1 Image

All ERK1/ERK2 Antibodies

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody Summary

Species Reactivity

Human

Specificity

Detects human ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.

Source

Monoclonal Mouse IgG₁ Clone # 798508

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the ERK1 T202/Y204 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human and Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 5 minutes and 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB1825) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-ERK1 (T202/Y204) and Phospho-ERK2 (T185/Y187) at approximately 43 and 41 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1; ERK-1; ERK1/ERK2; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAPK 1; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; p44erk1; p44-ERK1; p44mapk; p44-MAPK; PRKM3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

TGFbeta1-Smad canonical and -Erk noncanonical pathways participate in interleukin-17-induced epithelial-mesenchymal transition in Sjï¿½gren's syndrome
Authors: M Sisto, L Lorusso, G Ingravallo, D Ribatti, S Lisi
Lab. Invest., 2020-01-10;100(6):824-836.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot
Dysregulation of Mir-196b in Head and Neck Cancers Leads to Pleiotropic Effects in the Tumor Cells and Surrounding Stromal Fibroblasts
Authors: S Álvarez-Te, ST Menéndez, MÁ Villaronga, JP Rodrigo, L Manterola, L de Villala, JC de Vicente, L Alonso-Dur, MP Fernández, CH Lawrie, JM García-Ped
Sci Rep, 2017-12-19;7(1):17785.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Extremely low frequency electromagnetic fields affect proliferation and mitochondrial activity of human cancer cell lines
Authors: Michele Destefanis, Marta Viano, Christian Leo, Gianpiero Gervino, Antonio Ponzetto, Francesca Silvagno
International Journal of Radiation Biology