Human Phospho-MSK1 (S212) Antibody

Name: Human Phospho-MSK1 (S212) Antibody
Brand: R&D Systems
SKU: AF1036
Rating: 4.3 (3 reviews)

Catalog #: AF1036
2 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1036
AF1036-SP

Detection of Human Phospho-MSK1 (S212) by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-MSK1 (S212) Antibody Summary

Species Reactivity

Human

Specificity

Detects human MSK1 when phosphorylated at S212.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human MSK1 S212 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-MSK1 (S212) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM H₂O₂for 1 hour or 300 mM sorbitol for 30 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1 (S212) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1036), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1 (S212) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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MSK1 in Human Kidney Cancer Tissue. MSK1 phosphorylated at S212 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Rabbit Anti-Human Phospho-MSK1 (S212) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1036) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSK1

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name

Mitogen- and Stress-activated Protein Kinase 1

Entrez Gene IDs

9252 (Human); 73086 (Mouse)

Alternate Names

EC 2.7.11; MGC1911,90 kDa ribosomal protein S6 kinase 5; MSK1; MSK1EC 2.7.11.1; MSPK1; Nuclear mitogen- and stress-activated protein kinase 1; ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kD, polypeptide 5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLPK; RPS6KA5; RSKL; RSK-like protein kinase; S6K-alpha-5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-MSK1 (S212) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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ERK2 signaling regulates cell-cell adhesion of epithelial cells and enhances growth factor-induced cell scattering
Authors: J Rasl, J Grusanovic, Z Klimova, J Caslavsky, T Grousl, J Novotny, M Kolar, T Vomastek
Cellular Signalling, 2022-08-04;0(0):110431.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES cells.
Authors: Tamguney T, Zhang C, Fiedler D, Shokat K, Stokoe D
Exp. Cell Res., 2008-04-23;314(11):2299-312.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot