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Human Phospho-NFATC1 (S172) Antibody

Catalog #: MAB5640
3 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5640
MAB5640-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-NFATC1 (S172) Antibody Summary

Species Reactivity
Human
Specificity
Detects human NFATC1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 679340
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human S172 site.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Jurkat human acute T cell leukemia cell line treated with PMA and Ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC1

NFATC1 (Nuclear factor of activated T cells c1), also called NFAT2a, is a 120 kDa, 943 amino acid member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172 within a transactivation domain. Upon a rise in intracellular Ca++, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Multiple isoforms exist.

Long Name
Nuclear Factor of Activated T Cells C1
Entrez Gene IDs
4772 (Human); 18018 (Mouse)
Alternate Names
MGC138448; NFAT transcription complex cytosolic component; NFAT2; NFAT2cytoplasmic 1; NFATC; NF-ATC; NFATC1; NF-ATc1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

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Citations for Human Phospho-NFATC1 (S172) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. GSK3 inhibition with low dose lithium supplementation augments murine muscle fatigue resistance and specific force production
    Authors: Whitley KC, Hamstra SI, Baranowski RW et al.
    Physiol Rep
  2. Neurogranin is expressed in mammalian skeletal muscle and inhibits calcineurin signaling and myoblast fusion
    Authors: Fajardo VA, Watson CJF, Bott KN et al.
    American Journal of Physiology-Cell Physiology
  3. CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation
    Authors: J Deng, ES Wang, RW Jenkins, S Li, R Dries, K Yates, S Chhabra, W Huang, H Liu, AR Aref, E Ivanova, CP Paweletz, M Bowden, CW Zhou, GS Herter-Spr, JA Sorrentino, JE Bisi, PH Lizotte, AA Merlino, MM Quinn, LE Bufe, A Yang, Y Zhang, H Zhang, P Gao, T Chen, ME Cavanaugh, AJ Rode, E Haines, PJ Roberts, JC Strum, WG Richards, JH Lorch, S Parangi, V Gunda, GM Boland, R Bueno, S Palakurthi, GJ Freeman, J Ritz, WN Haining, NE Sharpless, H Arthanari, GI Shapiro, DA Barbie, NS Gray, KK Wong
    Cancer Discov, 2017-11-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human Phospho-NFATC1 (S172) Antibody

Average Rating: 4 (Based on 3 Reviews)

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Human Phospho-NFATC1 (S172) Antibody
By Nar Katuwal on 04/21/2021
Application: WB Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human
Western Blot Human Phospho-NFATC1 (S172) Antibody MAB5640
Human Phospho-NFATC1 (S172) Antibody
By Jignesh Ganeshbhai Patel on 09/10/2018
Application: WB Sample Tested: Blood monocytes,HeLa cells Species: Human
Human Phospho-NFATC1 (S172) Antibody
By Jignesh Patel on 09/10/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HeLa cells,THP-1 human acute monocytic leukemia cell line Species: Human