Home / RelA/NFkB p65 / Human Phospho-RelA/NFkB p65 (S536) Antibody

Human Phospho-RelA/NFkB p65 (S536) Antibody

Catalog #: MAB7226
5 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7226
MAB7226-SP
Detection of Human Phospho-RelA/NF kappa B p65 (S536) by Western Blot.
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Product Details
Citations (5)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-RelA/NFkB p65 (S536) Antibody Summary

Species Reactivity
Human
Specificity
Detects human RelA/NF kappa B p65 when phosphorylated at S536.
Source
Monoclonal Mouse IgG1 Clone # 817403
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the RelA/NF kappa B p65 S536 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-RelA/NF?B p65 (S536) antibody by Western Blot. View Larger

Detection of Human Phospho-RelA/NF kappa B p65 (S536) by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB7226) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-RelA/ NF kappa B p65 (S536) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry Phospho-RelA/NFkB p65 (S536) antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-RelA/NFkB p65 (S536) in HT‑29 Human Cell Line. RelA/NF kappa B p65 phosphorylated at S536 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line untreated (lower panel) or treated with Calyculin A (Catalog # 1336) and Recombinant Human TNF-a (Catalog # 210-TA; upper panel) using Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB7226) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RelA/NFkB p65

RelA belongs to a family of transcription factors (NF kappa B (nuclear factor kappa from B cells) complex) that play a fundamental role in inflammatory and immune responses. The NF kappa B complex is composed of a heterodimer of a Rel family member (RelA, c-Rel, RelB) and either NF kappa B1 or NF kappa B2 subunits. RelA and NF kappa B1 are the most common heterodimeric pair. The NF kappa B complex is sequestered in the cytoplasm by inhibitory I kappa B proteins. Upon cellular activation, the ubiquitin-proteosome pathway degrades the I kappa B proteins allowing the NF kappa B complex to translocate to the nucleus and activate gene transcription.

Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Entrez Gene IDs
5970 (Human)
Alternate Names
MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

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Citations for Human Phospho-RelA/NFkB p65 (S536) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. beta1-Integrin plays a major role in resveratrol-mediated anti-invasion effects in the CRC microenvironment
    Authors: A Brockmuell, AL Mueller, P Shayan, M Shakibaei
    Frontiers in Pharmacology, 2022-09-02;13(0):978625.
  2. Evidence That beta 1-Integrin Is Required for the Anti-Viability and Anti-Proliferative Effect of Resveratrol in CRC Cells
    Authors: Aranka Brockmueller, Parviz Shayan, Mehdi Shakibaei
    International Journal of Molecular Sciences
  3. Resveratrol Modulates Chemosensitisation to 5-FU via beta1-Integrin/HIF-1alpha Axis in CRC Tumor Microenvironment
    Authors: A Brockmuell, S Girisa, AB Kunnumakka, M Shakibaei
    International Journal of Molecular Sciences, 2023-03-05;24(5):.
  4. Calebin A targets the HIF-1?/NF-?B pathway to suppress colorectal cancer cell migration
    Authors: Brockmueller, A;Girisa, S;Motallebi, M;Kunnumakkara, AB;Shakibaei, M;
    Frontiers in pharmacology
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
    Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
    Molecular Medicine Reports, 2018-06-20;0(0):1791-3004.
    Species: Human
    Sample Types: Cell Lysate, Cell Lysates
    Applications: Western Blot

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Reviews for Human Phospho-RelA/NFkB p65 (S536) Antibody

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Human Phospho-RelA/NFkB p65 (S536) Antibody
By Anonymous on 12/25/2021
Application: WB Sample Tested: HeLa human cervical epithelial carcinoma cell line Species: Human
Western Blot Human Phospho-RelA/NFkB p65 (S536) Antibody MAB7226