Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody

Catalog #: MAB54361
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB54361-100
MAB54361-SP

Detection of Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ribosomal Protein S6 when dually phosphorylated at S235 and S236.

Source

Monoclonal Rat IgG_2A Clone # 973210

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing human Ribosomal Protein S6 S235/S236 sites
Accession # P62753

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 30 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-Ribosomal Protein S6/RPS6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospho-Ribosomal Protein S6/RPS6 (S235/S236) in MCF‑7 Human Cell Line. Ribosomal Protein S6/RPS6 was detected in immersion fixed MCF-7 human breast cancer cell line untreated or treated with Rapamycin using Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ribosomal Protein S6/RPS6

40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.

Entrez Gene IDs

6194 (Human); 20104 (Mouse); 29304 (Rat)

Alternate Names

Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Evaluation of the mTORC activity in the presence of Toxoplasma gondii and azathioprine in human monocyte cell line
Authors: S Nemati, H Mohammad R, A Meyfour, H Pazoki, H Asadzadeh, S Shahrokh, H Mirjalali
BMC microbiology, 2023-03-21;23(1):77.
Species: Human
Sample Types: Cell Lysates
Effect of gut microbiota on depressive-like behaviors in mice is mediated by the endocannabinoid system
Authors: G Chevalier, E Siopi, L Guenin-Mac, M Pascal, T Laval, A Rifflet, IG Boneca, C Demangel, B Colsch, A Pruvost, E Chu-Van, A Messager, F Leulier, G Lepousez, G Eberl, PM Lledo
Nature Communications, 2020-12-11;11(1):6363.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot