Human Phospho-STAT2 (Y690) Antibody

Detection of Human Phospho-STAT2 (Y690) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-STAT2 (Y690) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of STAT2 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN-aA (11100-1, filled histogram) for 20 minutes was stained with Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with 90% methanol.

Detection of Human Phospho-STAT2 (Y690) by Simple Western^TM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT2 (Y690) at approximately 111 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human STAT2 by Western Blot Induction of type I IFN signaling by viral and nonviral stimuli is inhibited in G2/M-arrested cells. Suit2 cells were treated for 25 h with the vehicle, paclitaxel, colchicine, or ruxolitinib at 500 nM. Cells were then treated with the vehicle (untreated), TransIT reagent (0.5%, vol/vol), poly(I:C) at 10 μg/ml plus TransIT reagent, IFN-alpha at 5,000 U/ml, or VSV at an MOI of 30 based on titration on BHK-21 cells. VSV was aspirated 1 h later, and medium was added to infected wells. Cells remained in treatment for a total of 4 h, after which total protein was isolated. Western blot results for STAT1 and -2 proteins and their phosphorylated forms are shown in addition to VSV proteins. GAPDH was used to confirm that protein loading was the same across the gel. Protein names and protein sizes in kilodaltons are indicated on the left and right, respectively. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30487274), licensed under a CC-BY license. Not internally tested by R&D Systems.