Human Phospho-STAT3 (S727) Antibody

Detection of Human Phospho-STAT3 (S727) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-STAT3 (S727) Monoclonal Antibody (Catalog # MAB4934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-STAT3 (S727) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-STAT3 (S727) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Mouse anti-Human Phospho-STAT3 (S727) Molyclonal Antibody (Catalog # MAB4934) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Detection of Human STAT3 by Western Blot Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes after exposure to increasing concentrations of IL-10; (c) STAT3-P inhibition of IL-10 (30 ng/mL) by Stattic.Bar plots show data from at least 3 independent experiments and the sho Western blot shows data from a representative experiment. M  =  media, iso ctrl  =  isotype control. * P<0.05, ***P<0.01 (Mann-Whitney test, compared with Media control). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24520384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human STAT3 by Western Blot Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes after exposure to increasing concentrations of IL-10; (c) STAT3-P inhibition of IL-10 (30 ng/mL) by Stattic.Bar plots show data from at least 3 independent experiments and the sho Western blot shows data from a representative experiment. M  =  media, iso ctrl  =  isotype control. * P<0.05, ***P<0.01 (Mann-Whitney test, compared with Media control). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24520384), licensed under a CC-BY license. Not internally tested by R&D Systems.