Home / STAT6 / Human Phospho-STAT6 (Y641) Antibody

Human Phospho-STAT6 (Y641) Antibody

Catalog #: AF3717
2 Citations Datasheet / COA / SDS
Newer Version Available: MAB3717
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Discontinued Product

AF3717 has been discontinued and is replaced by MAB3717.

Detection of STAT6 in Daudi Human Cell Line by Flow Cytometry.
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Citations (2)
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Human Phospho-STAT6 (Y641) Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT6 when phosphorylated at Y641.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human STAT6 Y641 site.
Formulation
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of STAT6 antibody in Daudi Human Cell Line antibody by Flow Cytometry. View Larger

Detection of STAT6 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 20 minutes, then stained with Rabbit Anti-Human Phospho-STAT6 (Y641) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3717, dark orange filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Western Blot Detection of Human Phospho-STAT6 (Y641) antibody by Western Blot. View Larger

Detection of Human Phospho-STAT6 (Y641) by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 30 minutes. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-STAT6 (Y641) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3717) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT6 (Y641) was detected at approximately 110 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 0.5 µg/mL Human STAT6 Monoclonal Antibody (lower panel, Catalog # MAB2167). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-STAT6 (Y641) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-STAT6 (Y641) by Simple WesternTM. Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 30 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT6 (Y641) at approximately 101 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-STAT6 (Y641) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3717). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Long Name
Signal Transducer and Activator of Transcription 6
Entrez Gene IDs
6778 (Human); 20852 (Mouse); 362896 (Rat)
Alternate Names
D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT

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Citations for Human Phospho-STAT6 (Y641) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. IL-4 Downregulates IL-1beta and IL-6 and Induces GATA3 in Psoriatic Epidermal Cells: Route of Action of a Th2 Cytokine.
    Authors: Onderdijk A, Baerveldt E, Kurek D, Kant M, Florencia E, Debets R, Prens E
    J Immunol, 2015-07-13;195(4):1744-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Opposing control of rhabdomyosarcoma growth and differentiation by myogenin and interleukin 4.
    Authors: Nanni P, Nicoletti G, Palladini A, Astolfi A, Rinella P, Croci S, Landuzzi L, Monduzzi G, Stivani V, Antognoli A, Murgo A, Ianzano M, De Giovanni C, Lollini PL
    Mol. Cancer Ther., 2009-04-01;8(4):754-61.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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