Which phosphorylated sites are recognized by this assay?

Name: Human Phospho-TrkB DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC688-2
Price: 470 USD

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-TrkB DuoSet IC ELISA

Catalog #: DYC688-2
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DYC688E
DYC688-5
DYC688-2

Ancillary Products Available

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

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Product Details

Citations (1)

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Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human Phospho-TrkB DuoSet IC ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

Cell lysates (100 µL)

Sufficient Materials

Kits available for two, five, or fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human TrkB in cell lysates. An immobilized capture antibody specific for TrkB binds both phosphorylated and unphosphorylated TrkB. After washing away unbound material, a HRP-conjugated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15-(96-well) plate pack sizes
Economical alternative to Western blot

Kit Content

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂O₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TrkB

Members of the Trk family (TrkA, B, and C) of receptor tyrosine kinases act as receptors for the neurotrophin family: BDNF, NGF, NT-3, and NT-4. Trks exhibit selectivity for specific neurotrophins. TrkA, TrkB and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a common structural organization consisting of an extracellular region with two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains, and an intracellular region that contains the tyrosine kinase domain. They are best known for their regulation of neuronal survival and axon outgrowth.

Long Name:

Neurotrophic Tyrosine Kinase Receptor B

Entrez Gene IDs:

4915 (Human); 18212 (Mouse); 25054 (Rat)

Alternate Names:

BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B

Citation for Human Phospho-TrkB DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
Int. J. Oncol., 2010-06-01;36(6):1469-76.
Species: Human
Sample Types: Cell Lysates

FAQs

Which phosphorylated sites are recognized by this assay?
- This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

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