Human PI 3-Kinase p110 delta Antibody

Detection of Human PI 3-Kinase p110δ by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110d Monoclonal Antibody (Catalog # MAB2687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p110 delta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human PI 3-Kinase p110 delta by Western Blot Inhibition of PI3Kδ and PI3K gamma subunits in MM proliferation. (a) Western blot analysis of PI3Kδ and PI3K gamma subunits in MM cell lines and MM primary cells. Blots were probed with beta -actin to show sample loading. (b) MM primary cells were treated with idelalisib (1 μm), CZC24832 (1 μm) and duvelisib (1 μm) for 72 h and then assessed for cell viability using CellTiter Glo. (c) MM primary cells were treated with idelalisib (1 μm), CZC24832 (1 μm) and duvelisib (1 μm) for 72 h and then assessed for apoptosis by PI/Annexin V staining (n=4). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/bcj201716), licensed under a CC-BY license. Not internally tested by R&D Systems.