Home / PI 3-Kinase p110 gamma / Human PI 3-Kinase p110 gamma Antibody

Human PI 3-Kinase p110 gamma Antibody

Catalog #: MAB2999
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2999
MAB2999-SP
Detection of Human PI 3-Kinase p110 gamma by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PI 3-Kinase p110 gamma Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human PI 3-Kinase p110 gamma in Western blots.
Source
Monoclonal Rat IgG2A Clone # 304103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PI 3-Kinase p110 gamma
Lys298-Leu467
Accession # P48736
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PI 3-Kinase p110? antibody by Western Blot. View Larger

Detection of Human PI 3-Kinase p110 gamma by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110 gamma Monoclonal Antibody (Catalog # MAB2999) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PI 3-Kinase p110 gamma at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p110 gamma

Class I phosphoinositide 3-kinases (PI 3-Kinases) are dual-specific lipid and protein kinases involved in numerous intracellular signaling pathways. Class IA PI 3‑Kinases (p110 alpha, beta  and δ) are typically activated through receptor tyrosine kinase signaling, whereas p110 gamma, the single member of class IB, is activated by seven‑transmembrane G‑protein‑coupled receptors (GPCRs). While p110 alpha and beta are ubiquitiously expressed, expression of p110 gamma is mainly restricted to cells of the hematopoietic system.

Long Name
Protein Tyrosine Phosphatase 1G
Entrez Gene IDs
5294 (Human); 30955 (Mouse); 298947 (Rat)
Alternate Names
1-phosphatidylinositol 3-kinase; EC 2.7.1; EC 2.7.1.153; p110-gamma; p120-PI3K; phosphatidylinositol 3 kinase gamma, p110 gamma; phosphatidylinositol 3-kinase catalytic 110-kD gamma; phosphatidylinositol 3-kinase, catalytic, gamma polypeptide; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform; phosphoinositide-3-kinase gamma catalytic subunit; phosphoinositide-3-kinase, catalytic, gamma polypeptide; PI 3Kinase p110 gamma; PI 3-Kinase p110 gamma; PI3CG; PI3K; PI3K-gamma ; PI3Kgamma; PI3K-gamma; PI3-kinase subunit gamma; PIK3; PIK3CG; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma

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Citations for Human PI 3-Kinase p110 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Phosphoinositide 3-kinase mediated signaling in lobster olfactory receptor neurons.
    Authors: Corey EA, Bobkov Y, Pezier A, Ache BW
    J. Neurochem., 2010-02-02;113(2):341-50.
    Species: Lobster
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. Nuclear factor-kappaB decoy oligodeoxynucleotides ameliorate impaired glucose tolerance and insulin resistance in mice with cecal ligation and puncture-induced sepsis.
    Authors: Matsuda N, Yamamoto S, Yokoo H, Tobe K, Hattori Y
    Crit. Care Med., 2009-10-01;37(10):2791-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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