Does AF7388 recognize all 3 isoforms (a, b, and c) of Human PITX2?

Name: Human PITX2 Antibody
Brand: R&D Systems
SKU: AF7388
Rating: 4.5 (2 reviews)

Yes, we would expect AF7388 to recognize all isoforms of Humaan PITX2 as the aa sequence used as the immunogen is present and unchanged in all three isoforms.

Human PITX2 Antibody

Catalog #: AF7388
8 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7388
AF7388-SP

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All PITX2 Antibodies

Product Details

Citations (8)

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PITX2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PITX2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human PITX1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PITX2
Ala149-Val317
Accession # Q99697

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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PITX2 in Human Thyroid Cancer Tissue. PITX2 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human PITX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7388) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PITX2

PITX2 (PITuitary homeoboX 2; also ARP1 and RIEG) is a 33-36 kDa nuclear member of the bicoid subfamily, paired homeobox family of transcription factors. It is widely expressed in the embryo, and serves as the earliest known marker for pituitary gland development. A long isoform commonly called PITX2C also plays a very key role in the establishment of left-right organ asymmetry. PITX2 appears in multiple germ layer derivatives, and it appears that one of its important functions is to integrating retinoic acid and Wnt/ beta -catenin signaling. The N- and C-terminal regions of PITX2 normally interact noncovalently, rendering PITX2 inactive. Over aa 149‑317, human and mouse PITX2 are identical in aa sequence.

Long Name

Pituitary Homeobox 2

Entrez Gene IDs

5308 (Human); 18741 (Mouse); 54284 (Rat)

Alternate Names

all1-responsive gene 1; ALL1-responsive protein ARP1; ARP1MGC20144; Brx1; Homeobox protein PITX2; IDG2; IGDS; IGDS2; IHG2; IRID2; Otlx2; paired-like homeodomain 2; Paired-like homeodomain transcription factor 2MGC111022; pituitary homeo box 2; pituitary homeobox 2; PITX2; PTX2; RGS; RGSRIEG1Brx1; rieg bicoid-related homeobox transcription factor 1; RIEG bicoid-related homeobox transcription factor; RIEG; RIEG1; RP1; RS; Solurshin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PITX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Wolf-Hirschhorn Syndrome Candidate 1 (Whsc1) Methyltransferase Signals Via a Pitx2-miR-23/24 Axis to Effect Tooth Development
Authors: Su, D;Eliason, S;Sun, Z;Shao, F;Amendt, BA;
The Journal of biological chemistry
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
The miR-200 family is required for ectodermal organ development through the regulation of the epithelial stem cell niche
Authors: Mason Sweat, Yan Sweat, Wenjie Yu, Dan Su, Riley J. Leonard, Steven L. Eliason et al.
Stem Cells
Tooth Removal in the Leopard Gecko and the de novo Formation of Replacement Teeth
Authors: KS Brink, JI Henríquez, TM Grieco, JR Martin Del, K Fu, JM Richman
Frontiers in Physiology, 2021-05-04;12(0):576816.
Species: Gecko
Sample Types: Whole Tissue
Applications: IHC
Cascade diversification directs generation of neuronal diversity in the hypothalamus
Authors: YH Zhang, M Xu, X Shi, XL Sun, W Mu, H Wu, J Wang, S Li, P Su, L Gong, M He, M Yao, QF Wu
Cell Stem Cell, 2021-04-21;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease
Authors: CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish
Cell Stem Cell, 2020-02-13;0(0):.
Species: Mouse, Rat
Sample Types: Whole Cells
Applications: ICC
Pitx2-Sox2-Lef-1 interactions specify progenitor oral/dental epithelial cell signaling centers
Authors: Wenjie Yu, Zhao Sun, Yan Sweat, Mason Sweat, Shankar Rengasamy Venugopalan, Steven Eliason et al.
Development
Isolation of LMX1a ventral midbrain progenitors improves the safety and predictability of human pluripotent stem cell-derived neural transplants in Parkinsonian Disease
Authors: IR de Luzy, JC Niclis, CW Gantner, JA Kauhausen, CPJ Hunt, C Ermine, CW Pouton, LH Thompson, CL Parish
J. Neurosci., 2019-10-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
Authors: Gantner C, Cota-Coronado A, Thompson L, Parish C
STAR Protoc

FAQs

Does AF7388 recognize all 3 isoforms (a, b, and c) of Human PITX2?
- Yes, we would expect AF7388 to recognize all isoforms of Humaan PITX2 as the aa sequence used as the immunogen is present and unchanged in all three isoforms.

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