Human PKC epsilon Antibody

Catalog #: MAB51341 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB51341-100
MAB51341-SP

Detection of Human PKC epsilon by Western Blot.

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All PKC epsilon Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PKC epsilon Antibody Summary

Species Reactivity

Human

Specificity

Detects human PKC epsilon in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 666843

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PKC epsilon
Gln580-Pro737
Accession # Q02156

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

5 µg/mL

Human hippocampus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PKC epsilon by Western Blot. Western blot shows lysates of Human brain (hippocampus) tissue and human brain (hypothalamus) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PKCe Monoclonal Antibody (Catalog # MAB51341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKCe at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human PKC epsilon by Simple Western^TM. Simple Western shows lysates of human hippocampus, loaded at 0.5 mg/ml. A specific band was detected for PKC epsilon at approximately 98 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human PKC epsilon Monoclonal Antibody (Catalog # MAB51341). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC epsilon

PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca⁺⁺‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca⁺⁺-binding plus lipid binding regulatory region (aa 1-99 and 169-292, respectively), and an ATP-binding catalytic domain (aa 408-668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates the enzyme and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118-737. Over aa 580-737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon, respectively.

Long Name

Protein Kinase C epsilon

Entrez Gene IDs

5581 (Human); 18754 (Mouse); 29340 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.13; MGC125656; nPKC-epsilon; PKC epsilon; PKCE; PKCEMGC125657; PRKCE; protein kinase C epsilon type; protein kinase C, epsilon