Human PLA2G16 Antibody

Catalog # Availability Size / Price Qty
AF6190-SP Ships in 5-7 Days25 ug / $149
AF6190 Contact us for Delivery Time.100 ug / $459

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Detection of Human PLA2G16 by Western Blot.
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Product Details
Citations (1)
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Human PLA2G16 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLA2G16 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PLA2G16
Asp12-Asp132
Accession # P53816
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PLA2G16 antibody by Western Blot. View Larger

Detection of Human PLA2G16 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PLA2G16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6190) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLA2G16 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLA2G16

PLA2G16 (Group 16 phospholipase A2; also HREV107 and AdPLA) is a Ca++‑dependent 17‑18 kDa member of the H‑rev107 family of molecules. It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids. The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels. This results in decreased hormone‑sensitive lipase activity and increased adiposity. Human PLA216 is 162 amino acids (aa) in length. It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa 134‑154. The enzymatic activity resides in His23 and Cys113. There is one potential isoform that shows a 20 aa substitution for the first four amino acids. Over aa 12 ‑ 132, human PLA2G16 shares 88% aa identity with mouse PLA2G16.

Long Name
Phospholipase A2, Group XVI
Entrez Gene IDs
11145 (Human); 225845 (Mouse); 24913 (Rat)
Alternate Names
Adipose-specific phospholipase A2; AdPLA; AdPLAHRASLS3; EC 3.1.1.4; group XVI phospholipase A2; HRAS-like suppressor 3Ca-independent phospholipase A1/2; HRASLS3; HREV107; H-REV107-1; HREV107-3adipose-specific PLA2; HREV107H-rev 107 protein homolog; MGC118754; phospholipase A2, group XVI; PLA2G16; Renal carcinoma antigen NY-REN-65

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Citation for Human PLA2G16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Targeting PLA2G16, a lipid metabolism gene, by Ginsenoside Compound K to suppress the malignant progression of colorectal cancer
    Authors: L Yang, L Zheng, X Xie, J Luo, J Yu, L Zhang, W Meng, Y Zhou, L Chen, D Ouyang, H Zhou, Z Tan
    Journal of advanced research, 2021-06-12;36(0):265-276.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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