Human PLA2G1B Antibody

Catalog #: MAB5018 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5018
MAB5018-SP

Detection of Human PLA2G1B by Western Blot.

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All PLA2G1B Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PLA2G1B Antibody Summary

Species Reactivity

Human

Specificity

Detects human PLA2G1B in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) PLA2G2A, rhPLA2G7, recombinant mouse (rm) PLA2G2A, or rmPLA2R1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 630823

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PLA2G1B
Met1-Ser148
Accession # NM_000928

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human PLA2G1B (Catalog # 5018‑PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PLA2G1B by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human PLA2G1B Monoclonal Antibody (Catalog # MAB5018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLA2G1B at approximately 17 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLA2G1B

PLA2G1B, also called Secretory Phopholipase A2, hydrolyses the sn-2 ester bond of phospholipids, including those in cell membranes, to generate non-esterified free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released into plasma and biological fluids on appropriate cell activation, especially during systemic inflammatory, autoimmune, or allergic disease. PLA2G1B is abundant in digestive organs but is also found elsewhere. Human PLA2G1B shares 78% and 76% amino acid sequence identity with mouse and rat PLA2G1B, respectively.

Long Name

Phospholipase A2 Group IB

Entrez Gene IDs

5319 (Human); 18778 (Mouse); 29526 (Rat)

Alternate Names

Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B; phospholipase A2; phospholipase A2, group IB (pancreas); PLA2; PLA2A; PLA2AMGC119834; PLA2EC 3.1.1.4; PLA2G1B; PPLA2; PPLA2MGC119835