Human Plexin A1 Antibody

Catalog #: MAB65361 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB65361-100
MAB65361-SP

Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Plexin A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Plexin A1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 708954

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Plexin A1
Tyr964-Leu1152
Accession # Q9UIW2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunocytochemistry

3-25 µg/mL

Immersion fixed MDA-MB-231 human breast cancer cell line and Daudi human Burkitt's lymphoma cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Goat anti-Mouse IgG secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

N/A

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Detection of Plexin A1 in MDA-MB-231 cells and Daudi cells. Plexin A1 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line (Positive) and absent in Daudi human Burkitt's lymphoma cell line (Negative) using Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A1

Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.

Entrez Gene IDs

5361 (Human); 18844 (Mouse)

Alternate Names

NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1