Human PLZF Antibody

Detection of Human PLZF by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human PLZF by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse PLZF by Western Blot Increased mTORC1 activity in germ cells from Lkb1 newborn cKO testis. (a) Western blot of testis proteins at different postnatal ages using specific antibodies against p-S6K1 (T389), p-rpS6 (S235/6), rpS6 and beta -tubulin. rpS6, and beta -tubulin were used as internal controls. (b) Expression of PI3K-, mTOR- and SPC-related markers by western blot in P8 testes from control and Lkb1 cKO mice. (c) Immunohistochemistry for VASA, GATA4, p-rpS6 in P8 control testis. (d) Immunohistochemistry for Lin28a and p-rpS6 in P8 control testis. Serial sections were used in order to compare cellular localizations of these markers in the same tubule. Asterisks, different distribution of Lin28a and p-rpS6 in the same tubule resulting in strong positive for Lin28a and weak staining for p-rpS6. Arrows, cells expressing both Lin28a and p-rpS6. (e) Immunohistochemistry for Plzf and p-rpS6 in control and cKO mice. (f) Mean number of Plzf- or p-rpS6-positive cells in each tubule (positive cells/total tubules). The data were shown as mean±S.E.M. of at least three replicates. *P<0.05. Scale bars=50 μm Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29022902), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse PLZF by Immunohistochemistry Depletion of SPCs in Lkb1 cKO mice. Seminiferous tubules from 5- (a) and 13- (b) week-old control and Lkb1 cKO mice (n=3) were used for immunostaining using specific antibodies against PCNA, gamma H2AX and Plzf. Scale bars=50 μm. Arrows, representative Plzf-positive cells in tubules. (c) Quantification of Plzf-positive germ cells in each tubule. Data are presented as mean±S.E.M. **P < 0. 01 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29022902), licensed under a CC-BY license. Not internally tested by R&D Systems.