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Human Podoplanin Antibody

Catalog #: AF3670
20 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3670
AF3670-SP
Detection of Human Podoplanin by Western Blot.
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Podoplanin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Podoplanin in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Podoplanin
Glu21-Lys123
Accession # Q86YL7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
MG-63 human osteosarcoma cell line and U-118-MG human glioblastoma/astrocytoma cell line
Flow Cytometry
2.5 µg/106 cells
A431 human epithelial carcinoma cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Podoplanin by Western Blot. Western Blot shows lysates of MG‑63 human osteosarcoma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/ml of Sheep Anti-Human Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Podoplanin at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry Podoplanin antibody in Human Uterus by Immunohistochemistry (IHC-P). View Larger

Podoplanin in Human Uterus. Podoplanin was detected in immersion fixed paraffin-embedded sections of human uterus using 2 µg/mL Sheep Anti-Human Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3670) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podoplanin

Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family (1‑2). Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207‑228) and a short cytoplasmic tail (aa 229‑238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation (2‑3). There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells (4‑5), and numerous tumors, including colorectal tumors (3), squamous cell carcinomas (4, 6), testicular seminoma (7), and brain tumors (8‑10). One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver (1). Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2) (2). Their association is dependent on sialic acid on O-glycans of Podoplanin (2). Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis (2). Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth (2).

References
  1. Zimmer, G. et al. (1999) Biochem. J. 341:277.
  2. Katsue-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  3. Kato, Y. et al. (2003) J. Biol. Chem. 278:51599.
  4. Schacht, V. et al. (2005) Am. J. Pathol. 166:913.
  5. Breiteneder-Geleff, S. et al. (1997) Am. J. Pathol. 151:1141.
  6. Kato, Y. et al. (2005) Tumour Biol. 26:195.
  7. Kato, Y. et al. (2004) Oncogene 23:8552.
  8. Mishima, K. et al. (2006) Acta Neuropathol. 111:563.
  9. Mishima, K. et al. (2006) Acta Neuropathol. 111:483.
  10. Kato, Y. et al. (2006) Biochem. Biophys. Res. Commun. 349:1301.
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Entrez Gene IDs
10630 (Human); 14726 (Mouse); 54320 (Rat)
Alternate Names
36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha

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Citations for Human Podoplanin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
    Authors: Kinchen J, Chen HH, Parikh K et al.
    Cell
  2. Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids
    Authors: Nikoli? MZ, Caritg O, Jeng Q et al.
    Elife
  3. Osteocyte‐Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model
    Authors: Arooj Munir, Janne Elin Reseland, Hanna Tiainen, Håvard Jostein Haugen, Pawel Sikorski, Emil Frang Christiansen et al.
    JBMR Plus
  4. Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes
    Authors: Rouaud, L;Baudin, L;Gautier-Isola, M;Van Meerbeeck, P;Feyereisen, E;Blacher, S;van Baren, N;Kridelka, F;Lucas, S;Noel, A;
    Cancers
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Organoid modeling of human fetal lung alveolar development reveals mechanisms of cell fate patterning and neonatal respiratory disease
    Authors: K Lim, APA Donovan, W Tang, D Sun, P He, JP Pett, SA Teichmann, JC Marioni, KB Meyer, AH Brand, EL Rawlins
    Cell Stem Cell, 2022-12-08;30(1):20-37.e9.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  6. Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling
    Authors: H Moriyama, J Endo, M Kataoka, Y Shimanaka, N Kono, Y Sugiura, S Goto, H Kitakata, T Hiraide, N Yoshida, S Isobe, T Yamamoto, K Shirakawa, A Anzai, Y Katsumata, M Suematsu, K Kosaki, K Fukuda, H Arai, M Sano
    Nature Communications, 2022-05-31;13(1):3013.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease
    Authors: TB Layton, L Williams, N Yang, M Zhang, C Lee, M Feldmann, G Trujillo, D Furniss, J Nanchahal
    Proceedings of the National Academy of Sciences of the United States of America, 2022-03-23;119(13):e2120336119.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: CyTof, IHC
  8. Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures
    Authors: K Gkatzis, P Panza, S Peruzzo, DY Stainier
    Elife, 2021-09-29;10(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  9. Decreased Lymphangiogenic Activities and Genes Expression of Cord Blood Lymphatic Endothelial Progenitor Cells (VEGFR3+/Pod+/CD11b+ Cells) in Patient with Preeclampsia
    Authors: H Kwon, JY Kwon, J Song, YS Maeng
    International Journal of Molecular Sciences, 2021-04-19;22(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Cellular census of human fibrosis defines functionally distinct stromal cell types and states
    Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
    Nat Commun, 2020-06-02;11(1):2768.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  11. Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis
    Authors: KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso
    BMC Musculoskelet Disord, 2019-07-27;20(1):344.
    Species: Canine, Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Bevacizumab Improves Achilles Tendon Repair in a Rat Model
    Authors: H Tempfer, A Kaser-Eich, C Lehner, R Gehwolf, S Korntner, N Kunkel, A Wagner, M Gruetz, LM Heindl, F Schroedl, A Traweger
    Cell. Physiol. Biochem., 2018-04-16;46(3):1148-1158.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  13. Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells
    Authors: MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields
    Nat Commun, 2018-03-05;9(1):948.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
    Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
    PLoS ONE, 2017-06-09;12(6):e0179257.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Quantitative Analysis of the Cellular Microenvironment of Glioblastoma to Develop Predictive Statistical Models of Overall Survival
    Authors: Jennifer M Munson
    J. Neuropathol. Exp. Neurol., 2016-12-01;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer.
    Authors: Puram Sidharth V, Tirosh Itay, Parikh Anuraag S et al.
    Cell
  17. Immunohistochemical quantification of partial-EMT in oral cavity squamous cell carcinoma primary tumors is associated with nodal metastasis
    Authors: Parikh AS, Puram SV, Faquin WC et al.
    Oral Oncol.
  18. Delineating the dynamic evolution from preneoplasia to invasive lung adenocarcinoma by integrating single-cell RNA sequencing and spatial transcriptomics
    Authors: Zhu J, Fan Y, Xiong Y et al.
    Experimental & molecular medicine
  19. Lymphangiogenesis and Lymph Node Metastasis in Oral Squamous Cell Carcinoma
    Authors: SATOMI ARIMOTO, TAKUMI HASEGAWA, DAISUKE TAKEDA, IZUMI SAITO, RIKA AMANO, MASAYA AKASHI et al.
    Anticancer Research
  20. Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles
    Authors: E Reátegui, KE van der Vo, CP Lai, M Zeinali, NA Atai, B Aldikacti, FP Floyd, A H Khankhel, V Thapar, FH Hochberg, LV Sequist, BV Nahed, B S Carter, M Toner, L Balaj, D T Ting, XO Breakefiel, SL Stott
    Nat Commun, 2018-01-12;9(1):175.

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Reviews for Human Podoplanin Antibody

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Human Podoplanin Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human Podoplanin Antibody AF3670
Human Podoplanin Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Human Podoplanin Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human