Human/Porcine/Canine CD90/Thy1 Antibody

Catalog #: AF2067
13 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2067
AF2067-SP

Detection of Human CD90/Thy1 by Western Blot.

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All CD90/Thy1 Antibodies

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Citations (13)

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Human/Porcine/Canine CD90/Thy1 Antibody Summary

Species Reactivity

Human, Porcine, Canine

Specificity

Detects human CD90/Thy1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD90 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD90/Thy1
Gln20-Ser141
Accession # P04216

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

2.5 µL/10⁶ cells

See below

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CD90/Thy1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue, human brain (hippocampus) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 22-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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CD90/Thy1 in BG01V Human Embyonic Stem Cells. CD90/Thy1 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry

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CD90/Thy1 in Human Prostate Cancer Tissue. CD90/Thy1 was detected in formalin fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

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CD90/Thy1 in Canine Mesenchymal Stem Cells. CD90/Thy1 was detected in immersion fixed canine mesenchymal stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry

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CD90/Thy1 in Porcine Mesenchymal Stem Cells. CD90/Thy1 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Flow Cytometry

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Detection of CD90/Thy1 in Human Mesenchymal Stem Cells by Flow Cytometry. Human mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by PE-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Flow Cytometry

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Detection of CD90/Thy1 in Porcine Mesenchymal Stem Cells by Flow Cytometry. Porcine mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by PE-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Flow Cytometry

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Detection of CD90/Thy1 in Canine Mesenchymal Stem Cells by Flow Cytometry. Canine mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by PE-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD90/Thy1

CD90 (also Thy1/Thymus cell antigen 1) is a 25-35 kDa glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed on neurons, ovarian follicular cells, endothelial cells, fibroblasts and circulating CD34⁺ stem cells, and appears to act as an adhesion molecule. CD90 is known to bind to integrins beta 2, beta 5 and beta 3, the latter often accompanied by additional binding to syndecan-4. In the postnatal nervous system, this inhibits neurite outgrowth while stabilizing newly formed axonal networks. In the vascular system, CD90 mediates the extravasation of leukocytes. And in lung, a CD90: alpha v beta 5 interaction inhibits the extracellular activation of latent TGF-beta. Mature human CD90 is a 111 amino acid (aa) GPI-linked protein (aa 20-130). It contains one V-type Ig-like domain (aa 20-126) with a heparin-binding motif (aa 56-60), and a GPI-anchor amidated cysteine at position 130. The integrin binding site consists of an Arg35LeuAsp37 tripeptide. CD90 apparently forms 50‑60 kDa homodimers and 150 kDa homomultimers. There is one potential isoform variant that contains a 12 aa substitution for aa 1-13. Over aa 20-141, human CD90 shares 64% aa identity with mouse CD90 and 84% or 83% with horse and dog respectively.

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Alternate Names

CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Porcine/Canine CD90/Thy1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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High Therapeutic and Esthetic Properties of Extracellular Vesicles Produced from the Stem Cells and Their Spheroids Cultured from Ocular Surgery-Derived Waste Orbicularis Oculi Muscle Tissues
Authors: Kyung Min Lim, Ahmed Abdal Dayem, Yujin Choi, Yoonjoo Lee, Jongyub An, Minchan Gil et al.
Antioxidants (Basel)
Development and Preliminary Testing of Porcine Blood-Derived Endothelial-like Cells for Vascular Tissue Engineering Applications: Protocol Optimisation and Seeding of Decellularised Human Saphenous Veins
Authors: Andrew Bond, Vito Bruno, Jason Johnson, Sarah George, Raimondo Ascione
International Journal of Molecular Sciences
Obesity defined molecular endotypes in the synovium of patients with osteoarthritis provides a rationale for therapeutic targeting of fibroblast subsets
Authors: Susanne N. Wijesinghe, Amel Badoume, Dominika E. Nanus, Archana Sharma‐Oates, Hussein Farah, Michelangelo Certo et al.
Clinical and Translational Medicine
Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton's Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles
Authors: Park, S;Kim, S;Lim, K;Shin, Y;Song, K;Kang, GH;Kim, DY;Shin, HC;Cho, SG;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytomery
wwEfficient improvement of the proliferation, differentiation, and anti-arthritic capacity of mesenchymal stem cells by simply culturing on the immobilized FGF2 derived peptide, 44-ERGVVSIKGV-53
Authors: Bin Lee, S;Abdal Dayem, A;Kmiecik, S;Min Lim, K;Sik Seo, D;Kim, HT;Kumar Biswas, P;Do, M;Kim, DH;Cho, SG;
Journal of advanced research
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Improved Wound Healing and Skin Regeneration Ability of 3,2'-Dihydroxyflavone-Treated Mesenchymal Stem Cell-Derived Extracellular Vesicles
Authors: S Kim, Y Shin, Y Choi, KM Lim, Y Jeong, AA Dayem, Y Lee, J An, K Song, SB Jang, SG Cho
International Journal of Molecular Sciences, 2023-04-09;24(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model
Authors: S Kurokawa, Y Hashimoto, S Funamoto, K Murata, A Yamashita, K Yamazaki, T Ikeda, K Minatoya, A Kishida, H Masumoto
PLoS ONE, 2021-07-22;16(7):e0254160.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC
Notch signalling drives synovial fibroblast identity and arthritis pathology
Authors: K Wei, I Korsunsky, JL Marshall, A Gao, GFM Watts, T Major, AP Croft, J Watts, PE Blazar, JK Lange, TS Thornhill, A Filer, K Raza, LT Donlin, CW Siebel, CD Buckley, S Raychaudhu, MB Brenner
Nature, 2020-04-22;582(7811):259-264.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Novel chitosan/agarose/hydroxyapatite nanocomposite scaffold for bone tissue engineering applications: comprehensive evaluation of biocompatibility and osteoinductivity with the use of osteoblasts and mesenchymal stem cells
Authors: P Kazimiercz, A Benko, M Nocun, A Przekora
Int J Nanomedicine, 2019-08-19;14(0):6615-6630.
Species: Canine
Sample Types: Whole Cells
Applications: ICC
Podoplanin regulates the migration of mesenchymal stromal cells and their interaction with platelets
Authors: LSC Ward, L Sheriff, JL Marshall, JE Manning, A Brill, GB Nash, HM McGettrick
J. Cell. Sci., 2019-02-25;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Pathological Remodeling of Mitral Valve Leaflets from Unphysiologic Leaflet Mechanics after Undersized Mitral Annuloplasty to Repair Ischemic Mitral Regurgitation
Authors: A Sielicka, EL Sarin, W Shi, F Sulejmani, D Corporan, K Kalra, VH Thourani, W Sun, RA Guyton, M Padala
J Am Heart Assoc, 2018-11-06;7(21):e009777.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC

Single Cell Sequencing of Induced Pluripotent Stem Cell Derived Retinal Ganglion Cells (iPSC-RGC) Reveals Distinct Molecular Signatures and RGC Subtypes
Authors: Gudiseva HV, Vrathasha V, He J Et al.
Genes (Basel)

Transplanted human induced pluripotent stem cells- derived retinal ganglion cells embed within mouse retinas and are electrophysiologically functional
Authors: Vrathasha V, Nikonov S, Bell B et al.
iScience