Human PPP2R4 Antibody

Catalog #: AF5929 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5929-100
AF5929-SP

Detection of Human PPP2R4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PPP2R4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PPP2R4 in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PPP2R4
Glu14-Asn323
Accession # Q15257

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PPP2R4 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U266 human myeloma cell line, and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PPP2R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5929) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PPP2R4 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PPP2R4

PPP2R4, also known as serine/threonine protein phosphatase 2A regulatory subunit B’, PR53 isoform, and PTPA, is a widely expressed member of the PTPA-type peptidyl prolyl isomerase family. Human PPP2R4 is 358 amino acids (aa) in length. Three splicing variants produce four isoforms for PPP2R4, and isoform 2 is the standard protein. Isoform 1, isoform 3 and isoform 4 have deletions corresponding to aa 73-107, 45-108, and 73-149 in isoform 2, respectively. Human PPP2R4 is 87% aa identical to mouse and rabbit PPP2R4. PPP2R4 reversibly stimulates the variable phosphotyrosyl phosphatase activity of the PP2A core heterodimer in the presence of ATP and Mg²⁺. It has been shown that p53 downregulates the expression of PPP2R4.

Long Name

Serine/threonine Protein Phosphatase 2A Regulatory Subunit B

Entrez Gene IDs

5524 (Human); 110854 (Mouse)

Alternate Names

EC 5.2.1.8; MGC2184; Phosphotyrosyl phosphatase activator; PP2A phosphatase activator; PP2A subunit B' isoform PR53; PP2A; PP2A, subunit B', PR53 isoform; PPP2R4; protein phosphatase 2A activator, regulatory subunit 4; PTPA; regulatory subunit B' (PR 53); serine/threonine-protein phosphatase 2A activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B'