Human Pregnancy Zone Protein/PZP Antibody

Catalog #: AF8280 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8280
AF8280-SP

Detection of Human Pregnancy Zone Protein/PZP by Western Blot.

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All Pregnancy Zone Protein/PZP Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pregnancy Zone Protein/PZP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pregnancy Zone Protein/PZP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human A2ML1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Pregnancy Zone Protein/PZP
Thr26-Val1482
Accession # P20742

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Pregnancy Zone Protein/PZP by Western Blot. Western blot shows human pregnancy serum. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Pregnancy Zone Protein/PZP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pregnancy Zone Protein/PZP at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pregnancy Zone Protein/PZP

Pregnancy zone protein (PZP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6) is a 360 kDa secreted homodimeric glycoprotein, PZP monomer contains a region of decisive functional importance i.e. the bait region. This region serves as a substrate to a variety of proteinases, and cleavage in this domain causes a conformational change of the molecule. Human PZP is a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins. Highest expression of human PZP is reported in liver, medium expression in ovary, heart and stomach. Also this protein is found in plasma. Over aa 26-1482, human PZP is 56% aa identical to mouse PZP.

Entrez Gene IDs

5858 (Human)

Alternate Names

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6; CPAMD6; Pregnancy Zone Protein; pregnancy-zone protein; PZP