Home / Presenilin-1 / Human Presenilin-1 N-Terminal Fragment Antibody

Human Presenilin-1 N-Terminal Fragment Antibody

Catalog #: AF149
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF149
AF149-SP
Presenilin‑1 in Human Brain.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Presenilin-1 N-Terminal Fragment Antibody Summary

Species Reactivity
Human
Specificity
Detects human PS-1 NTF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) PS-1 CTF (aa 298-407) and rhPS-2 NTF (aa 1-80) is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Presenilin-1
Lys1-Lys80
Accession # P49768
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Presenilin‑1 N-Terminal Fragment
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Presenilin‑1 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Presenilin‑1 in Human Brain. Presenilin-1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 15 µg/mL Goat Anti-Human Presenilin-1 N-Terminal Frag-ment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF149) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Presenilin-1

Presenilin-1 (PS-1) and Presenilin-2 (PS-2) are integral membrane proteins that contain multiple transmembrane domains. Both are predominantly located within the endoplasmic reticulum (ER) and early Golgi apparatus. They are primarily expressed in neurons and are ubiquitously expressed in the brain. PS-1 and PS-2 are proteolytically cleaved to generate an N-terminal fragment (NTF) and a C-terminal fragment (CTF). Mutations in the gene for Presenilin may play an important role in the pathology of Alzheimer's disease.

Entrez Gene IDs
5663 (Human)
Alternate Names
AD3EC 3.4.23.-; Alzheimer disease 3; EC 3.4.23; FAD; presenilin 1; Presenilin1; Presenilin-1; Protein S182; PS-1; PS1presenilin-1; PSEN1; PSNL1; S182

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Citations for Human Presenilin-1 N-Terminal Fragment Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Erlin-2 is associated with active gamma-secretase in brain and affects amyloid beta-peptide production.
    Authors: Teranishi Y, Hur J, Gu G, Kihara T, Ishikawa T, Nishimura T, Winblad B, Behbahani H, Kamali-Moghaddam M, Frykman S, Tjernberg L
    Biochem Biophys Res Commun, 2012-07-04;424(3):476-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Proximity Ligation Assay (PLA)
  2. Presenilins, Notch dose control the fate of pancreatic endocrine progenitors during a narrow developmental window.
    Authors: Cras-Meneur C, Li L, Kopan R, Permutt MA
    Genes Dev., 2009-09-01;23(17):2088-101.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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