Home / Angiopoietin-like Protein 4/ANGPTL4 / Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody

Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody

Catalog #: AF3485
15 Citations Datasheet / COA / SDS
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AF3485
AF3485-SP
Detection of Human/Primate Angiopoietin‑like Protein 4/ANGPTL4 by Simple WesternTM.
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Citations (15)
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Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human ANGPTL4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ANGPTL3, rhAngiopoietin-1, -2, -3, and -4 is observed. In Western blots, approximately 1% cross-reactivity with rhANGPLT4 N-terminal Fragment is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 4/ANGPLT4
Leu165-Ser406
Accession # Q9BY76
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Angiopoietin‑like 4/ANGPTL4 (Catalog # 4487-AN)
Simple Western
50 µg/mL
Human Diabetic Adipose

Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate Angiopoietin‑like Protein 4/ANGPTL4 Biotinylated Antibody (Catalog # BAF3485)

Standard: Recombinant Human Angiopoietin-like 4 Protein, CF (Catalog # 4487-AN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western View Larger

Detection of Human/Primate Angiopoietin‑like Protein 4/ANGPTL4 by Simple WesternTM. Simple Western lane view shows lysates of Human Diabetic Adipose, loaded at 0.2 mg/mL. A specific band was detected for Angiopoietin‑like Protein 4/ANGPTL4 at approximately 59 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Primate Angiopoietin‑like Protein 4/ANGPTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3485). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 4/ANGPTL4

ANGPTL4, also known as PPAR gamma angiopoietin-related protein (PGAR), hepatic fibrinogen/angiopoietin-related protein (HFARP) and fasting-induced adipose factor (FIAF), is a secreted protein that shares structural homology with angiopoietins. It contains an N-terminal coiled-coil region that mediates covalent homo-oligomerization and a C-terminal fibrinogen-like domain. ANGPTL4 undergoes proteolytic processing and releases the C-terminal domain, which circulates as a monomer. At least 2 additional splice isoforms exist. ANGPTL4 is most highly expressed in adipose tissues. Its expression is up-regulated in endothelial cells and cardiomyocytes during hypoxia. ANGPTL4 is involved in the regulation of lipid and glucose metabolism. It has also been associated with angiogenesis. The amino acid sequence of human ANGPTL4 is 81%, 76% and 81% identical to that of porcine, mouse and canine ANGPTL4, respectively.

Entrez Gene IDs
51129 (Human); 57875 (Mouse); 362850 (Rat)
Alternate Names
Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein

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Citations for Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Angiopoietin-like protein 8 differentially regulates ANGPTL3 and ANGPTL4 during postprandial partitioning of fatty acids[S]
    Authors: Yan Q. Chen, Thomas G. Pottanat, Robert W. Siegel, Mariam Ehsani, Yue-Wei Qian, Eugene Y. Zhen et al.
    Journal of Lipid Research
  2. ANGPTL4 Regulates Psoriasis via Modulating Hyperproliferation and Inflammation of Keratinocytes
    Authors: Y Zuo, L Dai, L Li, Y Huang, X Liu, X Liu, X Duan, S Jiang, GM Deng, H Chen
    Frontiers in Pharmacology, 2022-07-04;13(0):850967.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Metformin Mitigates Nickel-Elicited Angiopoietin-Like Protein 4 Expression via HIF-1&alpha for Lung Tumorigenesis
    Authors: YT Kang, WC Hsu, CC Ou, HC Tai, HT Hsu, KT Yeh, JL Ko
    Int J Mol Sci, 2020-01-17;21(2):.
    Species: Human
    Sample Types: Cell Lys
    Applications: Western Blot
  4. Effect of diet-induced weight loss on angiopoietin-like protein 4 and adipose tissue lipid metabolism in overweight and obese humans
    Authors: BW van der Ko, RG Vink, JWE Jocken, NJT Roumans, GH Goossens, ECM Mariman, MA van Baak, EE Blaak
    Physiol Rep, 2018-07-01;6(13):e13735.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  5. Plasma angiopoietin-like 4 is related to phospholipid transfer protein activity in diabetic and non-diabetic subjects: role of enhanced low grade inflammation
    Authors: EG Gruppen, S Kersten, RPF Dullaart
    Lipids Health Dis, 2018-03-27;17(1):60.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  6. Regulation of angiopoietin-like 4 and lipoprotein lipase in human adipose tissue
    Authors: W Dijk, S Schutte, EO Aarts, IMC Janssen, L Afman, S Kersten
    J Clin Lipidol, 2018-02-17;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  7. Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels
    Authors: L Zhou, Y Huang, H Xie, X Mei
    Mol Med Rep, 2017-12-19;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines
    Authors: K Baba, Y Kitajima, S Miyake, J Nakamura, K Wakiyama, H Sato, K Okuyama, H Kitagawa, T Tanaka, M Hiraki, K Yanagihara, H Noshiro
    Sci Rep, 2017-09-11;7(1):11127.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. ANGPTL4 promotes the progression of cutaneous melanoma to brain metastasis
    Authors: S Izraely, S Ben-Menach, O Sagi-Assif, T Meshel, DM Marzese, S Ohe, I Zubrilov, M Pasmanik-C, DSB Hoon, IP Witz
    Oncotarget, 2017-07-05;8(44):75778-75796.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Short-chain fatty acids stimulate angiopoietin-like 4 synthesis in human colon adenocarcinoma cells by activating peroxisome proliferator-activated receptor gamma.
    Authors: Alex S, Lange K, Amolo T, Grinstead J, Haakonsson A, Szalowska E, Koppen A, Mudde K, Haenen D, Al-Lahham S, Roelofsen H, Houtman R, van der Burg B, Mandrup S, Bonvin A, Kalkhoven E, Muller M, Hooiveld G, Kersten S
    Mol Cell Biol, 2013-01-22;33(7):1303-16.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  11. Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids.
    Authors: Kersten S, Lichtenstein L, Steenbergen E, Mudde K, Hendriks HF, Hesselink MK, Schrauwen P, Muller M
    Arterioscler. Thromb. Vasc. Biol., 2009-04-02;29(6):969-74.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. Knockdown of angiopoietin-like 4 inhibits the development of human gastric cancer
    Authors: Jin-Wu Chen, Ying-Jia Luo, Zheng-Fei Yang, Li-Qiang Wen, Lin Huang
    Oncology Reports
  13. Regulation and clinical significance of the hypoxia-induced expression of ANGPTL4 in gastric cancer.
    Authors: Kubo H, Kitajima Y, Kai K et al.
    Oncol Lett
  14. Inflammation increases plasma angiopoietin-like protein 4 in patients with the metabolic syndrome and type 2 diabetes
    Authors: Nathanja Tjeerdema, Anastasia Georgiadi, Jacqueline T Jonker, Marjolijn van Glabbeek, Reza Alizadeh Alizadeh Dehnavi, Jouke T Tamsma et al.
    BMJ Open Diabetes Research & Care
  15. ANGPTL4 mediates shuttling of lipid fuel to brown adipose tissue during sustained cold exposure
    Authors: Wieneke Dijk, Markus Heine, Laurent Vergnes, Mariëtte R Boon, Gert Schaart, Matthijs K C Hesselink et al.
    eLife

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