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Human/Primate GDF-15 Biotinylated Antibody

Catalog #: BAF940
9 Citations 2 Reviews Datasheet / COA / SDS
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BAF940
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (2)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate GDF-15 Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human GDF-15 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human GDF‑11, recombinant mouse (rm) GDF-5, rmGDF-6, rmGDF-7, and rmGDF-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GDF-15 (R&D Systems, Catalog # 957-GD)
Ala197-Ile308
Accession # Q99988
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GDF‑15 (Catalog # 957-GD)

Human/Primate GDF-15 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate GDF‑15 Antibody (Catalog # MAB957)

Standard: Recombinant Human GDF-15 (CHO-expressed) Protein (Catalog # 957-GD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-15

Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.

References
  1. Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
  2. Böttner, M. et al. (1999) Gene 237:105.
  3. Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
  4. Fairlie, W.D. et al. (2001) J B.C 20:16911.
  5. Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
  6. Strelau, J. et al. (2000) J. Neurosci. 20:8597.
  7. Schober, A. et al. (2001) J. Comp. Neurol. 439:32.
Long Name
Growth Differentiation Factor 15
Entrez Gene IDs
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

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Citations for Human/Primate GDF-15 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Hyperbaric Oxygen Exposure Attenuates Circulating Stress Biomarkers: A Pilot Interventional Study
    Authors: Jae Seung Chang, Eunha Chang, Yoonsuk Lee, Yong Sung Cha, Seung-Kuy Cha, Won Gil Cho et al.
    International Journal of Environmental Research and Public Health
  2. Circulating levels of GDF15 in patients with myalgic encephalomyelitis/chronic fatigue syndrome
    Authors: A Melvin, E Lacerda, HM Dockrell, S O'Rahilly, L Nacul
    J Transl Med, 2019-12-04;17(1):409.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  3. GDF15 promotes osteosarcoma cell migration and invasion by regulating the TGF‑ beta signaling pathway
    Authors: Guangfu Chen, Min Wang, Xiang Liu
    Molecular Medicine Reports
  4. Glyceryl trinitrate‑induced cytotoxicity of docetaxel‑resistant prostatic cancer cells is associated with differential regulation of clusterin
    Authors: Sarra Bouaouiche, Lea Magadoux, Lucile Dondaine, Sylvie Reveneau, Nicolas Isambert, Ali Bettaieb et al.
    International Journal of Oncology
  5. GDF15/MIC1 and MMP9 Cerebrospinal Fluid Levels in Parkinson's Disease and Lewy Body Dementia.
    Authors: Walter Maetzler, Willy Deleersni, Valérie Hanssens, Alice Bernard, Kathrin Brockmann, Justus Marquetan, Isabel Wurster, Tim W Rattay, Lorenzo Roncoroni, Eva Schaeffer, Stefanie Lerche, Anja Apel, Christian Deuschle, Daniela Berg
    PLoS ONE, 2016-03-03;0(0):1932-6203.
    Species: Human
    Sample Types: Beads
    Applications: Luminex Development
  6. Macrophage inhibitory cytokine 1 biomarker serum immunoassay in combination with PSA is a more specific diagnostic tool for detection of prostate cancer.
    Authors: Li, Ji, Veltri, Robert W, Yuan, Zhen, Christudass, Christhu, Mandecki, Wlodek
    PLoS ONE, 2015-04-08;10(4):e0122249.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  7. AF1q: A Novel Mediator of Basal and 4-HPR-Induced Apoptosis in Ovarian Cancer Cells.
    Authors: Tiberio P, Cavadini E, Callari M
    PLoS ONE, 2012-06-26;7(6):e39968.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Growth differentiation factor-15 predicts mortality and morbidity after cardiac resynchronization therapy.
    Authors: Foley PW, Stegemann B, Ng K, Ramachandran S, Proudler A, Frenneaux MP, Ng LL, Leyva F
    Eur. Heart J., 2009-08-07;30(22):2749-57.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  9. Growth differentiation factor-15 as a prognostic marker in patients with acute myocardial infarction.
    Authors: Khan SQ, Ng K, Dhillon O, Kelly D, Quinn P, Squire IB, Davies JE, Ng LL
    Eur. Heart J., 2009-01-23;30(9):1057-65.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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Reviews for Human/Primate GDF-15 Biotinylated Antibody

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Human/Primate GDF-15 Biotinylated Antibody
By Anonymous on 11/08/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the detection in an ELISA along with MAB957 as the capture antibody. Human serum and plasma samples were measurable.

ELISA Human/Primate GDF-15 Biotinylated Antibody BAF940
Human/Primate GDF-15 Biotinylated Antibody
By Anonymous on 06/11/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the pair to MAB957 in an GDF-15 immunoassay.

ELISA Human/Primate GDF-15 Biotinylated Antibody BAF940