Human/Primate Ig Kappa Light Chain Antibody

Catalog #: MAB100502 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB100502-100
MAB100502-SP

Detection of Human and Primate Ig Kappa Light Chain by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate Ig Kappa Light Chain Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human Ig Kappa Light Chain in direct ELISAs. Detects human and primate Ig Kappa Light Chain in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2495C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human Ig Kappa Light Chain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Primate Ig Kappa Light Chain by Western Blot. Western blot shows human kappa light chain protein, human lambda light chain protein (negative control), human serum, primate serum, mouse serum (negative control), and rat serum (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Primate Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100502) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ig Kappa Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ig Kappa Light Chain

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain. Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Long Name

Immunoglobulin Kappa Light Chain

Alternate Names

ig kappa chain c region;Immunoglobulin K;immunoglobulin kappa light chain;Kappa LC; Ig Kappa Light Chain