Human Pro Caspase-10 Antibody

Catalog #: AF6864 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6864
AF6864-SP

Detection of Human Pro Caspase‑10 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pro Caspase-10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pro Caspase-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Caspase-2, rhCaspase-3 (long and short forms), rhCaspase-3, rhCaspase-7, rhCaspase-8, rhCaspase-8 p10, rhCaspase-8 p20 and rhCaspase-9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Pro Caspase-10
Met1-Pro228
Accession # Q92851

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Pro Caspase‑10 by Western Blot. Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line, human heart tissue, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Pro Caspase-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pro Caspase-10 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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Pro Caspase‑10 in Jurkat Human Cell Line. Pro Caspase-10 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human Pro Caspase-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6864) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-10

Caspase-10 (Cysteine-aspartic acid protease 10, FLICE2 and Mch4) is a 28-29 kDa member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on procaspases-3, 4, 6, 7, and 9. Human procaspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43 generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.

Entrez Gene IDs

843 (Human)

Alternate Names

Apoptotic protease Mch-4; CASP10; CASP-10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; Caspase10; Caspase-10; EC 3.4.22.63; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; Mch4; MCH4ALPS2