Human Pro-Relaxin‑3 Antibody Summary
Ala27-Cys142. Cross-reactivity with mature Relaxin-3 was removed.
Accession # Q8WXF3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Pro-Relaxin‑3 in Mouse Testis. Pro-Relaxin-3 was detected in immersion fixed frozen sections of adult mouse testis using Goat Anti-Human Pro-Relaxin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3107) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Relaxin-3
Human Relaxin-3 (H3 relaxin, INSL7) is one of seven relaxin-like peptides belonging to the insulin superfamily (1-4). Unlike human relaxins 1 and 2, it does not play a role in reproduction but appears to be a neuropeptide involved in stress response in the brain stem (3-5). The 142 amino acid (aa) Relaxin-3 pre-proprotein is processed to remove a 25 aa signal peptide and a connecting peptide (aa 53-118). The resulting mature Relaxin-3 is a 5.5 kDa, 51 aa secreted heterodimer of A (aa 119-142) and B (aa 26-52) peptides connected by two intermolecular disulfide bonds (1). Relaxin-3 is the only known ligand for the G-protein-coupled receptor GPCR135, designated RXFP3 (4, 6). In rodents, GPCR135 is expressed primarily in the supraoptic and paraventricular nucleus (6). This region has connections to the dorsal tegmental region of the pons (also called the nucleus incertus), where expression of Relaxin-3 is highest (5). Relaxin-3 also binds the more widely-expressed LGR7 (RXFP1) receptor, but with lower affinity than that of Relaxin-2 (1, 7). Although binding of Relaxin-3 to LGR7 increases intracellular cAMP, binding to GPCR135 inhibits cAMP accumulation, indicating coupling to Gi, Go or Gz by this receptor (1, 5). Relaxin-3 expression does not overlap well with its other receptor, GPCR142, which instead appears to be the primary receptor for INSL5 (3, 8).
- Kizawa, H. et al. (2003) Regul. Pept. 113:79.
- Wilkinson, T.N. et al. (2005) BMC Evol. Biol. 5:14.
- Bathgate, R.A. et al. (2006) Pharmacol. Rev. 58:7.
- Liu, C. et al. (2005) Ann. N.Y. Acad. Sci. 1041:47.
- Tanaka, M. et al. (2005) Eur. J. Neurosci. 21:1659.
- Liu, C. et al. (2003) J. Biol. Chem. 278:50754.
- Rosengren, K.J. et al. (2006) J. Biol. Chem. 281:5845.
- Liu, C. et al. (2003) J. Biol. Chem. 278:50765.
Product Datasheets
Citation for Human Pro-Relaxin‑3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus
Authors: L Lu, Y Ren, T Yu, Z Liu, S Wang, L Tan, J Zeng, Q Feng, R Lin, Y Liu, Q Guo, M Luo
Nat Commun, 2020-01-14;11(1):262.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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