Human Prokineticin R1/PKR1 Antibody

Catalog #: MAB4655 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4655
MAB4655-SP

Detection of Prokineticin R1/PKR1 in Human Monocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Prokineticin R1/PKR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Prokineticin R1/PKR1. Stains human Prokineticin R1/PKR1 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 420849

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Prokineticin R1/PKR1
Met1-Lys393
Accession # Q8TCW9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Prokineticin R1/PKR1 in Human Monocytes by Flow Cytometry. Human blood‑derived monocytes were stained with Human Prokineticin R1/PKR1 Monoclonal Antibody (Catalog # MAB4655, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prokineticin R1/PROKR1

Prokineticin Receptor 1 (PKR1), also called ZAQ or GPR73a (G-protein coupled receptor 73a), is a 7-transmembrane glycoprotein of the GPCR family. The extracellular portions of human PKR1 share 81% and 78% aa identity with corresponding portions of mouse PKR1 and human PKR2, with non-identity mainly in the N‑terminal sequences. Both PKR1 and PKR2 mediate the effects of prokineticins 1 and 2. This includes mitogenic regulation of angiogenesis in endocrine glands and stimulation of contraction in gastrointestinal smooth muscle.

Long Name

Prokineticin Receptor 1

Entrez Gene IDs

10887 (Human); 58182 (Mouse); 192648 (Rat)

Alternate Names

G protein-coupled receptor 73; G protein-coupled receptor ZAQ; GPR73; GPR73a; GPR73aG-protein coupled receptor 73; PKR1; PK-R1; PKR1G-protein coupled receptor ZAQ; Prokineticin R1; prokineticin receptor 1; ProkineticinR1; PROKR1; ZAQ