Human Prostasin/Prss8 Antibody

Catalog #: AF4599
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4599
AF4599-SP

Detection of Human Prostasin/Prss8 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Prostasin/Prss8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Prostasin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse Prostasin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Prostasin
Ala33-Gly319
Accession # Q16651

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Prostasin/Prss8 (Catalog # 4599-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Prostasin/Prss8 by Western Blot. Western blot shows lysates of human prostate tissue, LNCaP human prostate cancer cell line, PC-3 human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostasin/Prss8 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Prostasin/Prss8 in Human Prostate Cancer Tissue. Prostasin/Prss8 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 10 µg/mL Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human Prostasin/Prss8 by Simple Western^TM. Simple Western lane view shows lysates of human prostate tissue and LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Prostasin/Prss8 at approximately 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prostasin/Prss8

Prostasin, also known as channel activating protease 1, is a serine protease with trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of Prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of Prostasin is involved in the regulation of epithelial sodium channels (4, 5).

References

Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
Yu, J.X. et al. (1994) J. Biol. Chem. 269:18843.
Yu, J.X. et al. (1995) J. Biol. Chem. 270:13483.
Caldwell, R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C194.
Tong, Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.

Entrez Gene IDs

5652 (Human); 76560 (Mouse)

Alternate Names

CAP1; channel-activating protease 1; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.120; Prostasin; protease, serine, 8; Prss8; Serine protease 8

Product Datasheets

Product Datasheet

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Citation for Human Prostasin/Prss8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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