Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

Catalog #: MAB64371 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB64371
MAB64371-SP

Protein O‑Glucosyltransferase 1/POGLUT in Human Liver.

1 Image

All Protein O-Glucosyltransferase 1/POGLUT1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protein O‑Glucosyltransferase 1/POGLUT in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 737512

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Protein O‑Glucosyltransferase 1/POGLUT
Arg24-Leu388
Accession # Q8NBL1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Protein O‑Glucosyltransferase 1/POGLUT in Human Liver. Protein O-Glucosyltransferase 1/POGLUT was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Protein O-Glucosyltransferase 1/POGLUT Monoclonal Antibody (Catalog # MAB64371) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein O-Glucosyltransferase 1/POGLUT1

Protein O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). POGLUT1 has hydrolase activity on UDP-Glc in a phosphatase-coupled glycosyltransferase assay (4).

References

Acar, M. et al. (2008) Cell 132:247.
Teng, Y. et al. (2006) Gene 371:7
Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
Wu, Z.L. et al. (2011) Glycobiology 21:727.

Entrez Gene IDs

56983 (Human)

Alternate Names

9630046K23Rik; C3orf9; CAP10-like protein, 46 kDa; chromosome 3 open reading frame 9; CLP46; EC 2.4.1.-; hCLP46CAP10-like 46 kDa protein; KDELC family like 1; KDELCL1; KTEL (Lys-Tyr-Glu-Leu) containing 1; KTEL motif-containing protein 1; KTELC1; MDS010; MDSRP; MDSRPKTELC1; MGC32995; Myelodysplastic syndromes relative protein; POGLUT1; protein O-glucosyltransferase 1; Rumi; x 010 protein