Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Catalog #: MAB61341 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB61341
MAB61341-SP

Proteinase 3/Myeloblastin/PRTN3 in Human Granulocytes.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Proteinase 3/Myeloblastin/PRTN3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Proteinase 3/Meyeloblastin/PRTN3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 684022

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Proteinase 3/Meyeloblastin/PRTN3
Ala26-Arg249
Accession # P24158

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Proteinase 3/Myeloblastin/PRTN3 in Human Granulocytes. Proteinase 3/ Myeloblastin/PRTN3 was detected in immersion fixed human granulocytes using Mouse Anti-Human Proteinase 3/ Myeloblastin/PRTN3 Monoclonal Antibody (Catalog # MAB61341) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proteinase 3/Myeloblastin/PRTN3

Leukocyte proteinase 3 (PRTN3 or PR3), also called Myeloblastin (MBN) or NP-4, is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human MBN proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C-terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human MBN shares 68% aa sequence identity with mouse MBN.

Entrez Gene IDs

5657 (Human); 19152 (Mouse); 314615 (Rat)

Alternate Names

ACPA; AGP7; AGP7PR3; azurophil granule protein 7; C-ANCA; EC 3.4.21; EC 3.4.21.76; Leukocyte proteinase 3; MBN; MBT; Myeloblastin; Neutrophil proteinase 4; NP4; NP-4; P29C-ANCA antigen; PR-3CANCA; Proteinase 3; PRTN3; serine proteinase, neutrophil; Wegener autoantigen; Wegener granulomatosis autoantigen